A universal method for the preparation of covalent protein-DNA conjugates for use in creating protein nanostructures

被引：85

作者：

Duckworth, Benjamin P.

Chen, Yan

Wollack, James W.

Sham, Yuk

Mueller, Joachim D.

Taton, T. Andrew

Distefano, Mark D. ^{[1
]}

机构：

[1] Univ Minnesota, Dept Chem, Minneapolis, MN 55455 USA

[2] Univ Minnesota, Dept Phys, Minneapolis, MN 55455 USA

[3] Univ Minnesota, Minnesota Supercomp Inst, Minneapolis, MN 55455 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 46期

关键词：

click reactions; enzymes; nanostructures; protein labeling; protein structures;

D O I：

10.1002/anie.200701942

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Protein patterning: A protein, selectively labeled with an azide tag, was reacted with alkyne-functionalized DNA. These protein-DNA conjugates were used to create a DNA-assembled protein nanostructure. This method should facilitate the preparation of a range of protein-DNA hybrid structures with useful structural and functional features. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：8819 / 8822

页数：4

共 43 条

[31] OLIGONUCLEOTIDE-DIRECTED SELF-ASSEMBLY OF PROTEINS - SEMISYNTHETIC DNA STREPTAVIDIN HYBRID MOLECULES AS CONNECTORS FOR THE GENERATION OF MACROSCOPIC ARRAYS AND THE CONSTRUCTION OF SUPRAMOLECULAR BIOCONJUGATES [J].