Engineering novel restriction endonucleases: Principles and applications

被引：39

作者：

Jeltsch, A

Wenz, C

Wende, W

Selent, U

Pingoud, A

机构：

来源：

TRENDS IN BIOTECHNOLOGY | 1996年 / 14卷 / 07期

关键词：

D O I：

10.1016/0167-7799(96)10030-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Restriction endonucleases cleave DNA with remarkable sequence specificity. In this review, we summarize the status of, and prospects for, engineering restriction endonucleases with new specificities. Such variants could be of considerable commercial value because restriction enzymes are among the most frequently used enzymes in molecular biology, and not all the desirable specificities are available. While it has not yet been possible to effect specificity changes, mutants have been described that (1) exhibit relaxed specificity, (2) favour modified substrates over their natural substrates, (3) discriminate between cleavage sites located in different sequences, (4) prefer metal ions other than Mg2+ as cofactors for cleavage, or (5) possess site-specific DNA-nicking activity.

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页码：235 / 238

页数：4

相关论文

共 34 条

[11] ALTERING SPECIFICITY OF RESTRICTION ENDONUCLEASE - EFFECT OF REPLACING MG2+ WITH MN2+ [J].

HSU, MT ;

BERG, P .

BIOCHEMISTRY, 1978, 17 (01) :131-138

[12] MUTATIONAL ANALYSIS OF THE FUNCTION OF GLN115 IN THE ECORI RESTRICTION ENDONUCLEASE, A CRITICAL AMINO-ACID FOR RECOGNITION OF THE INNER THYMIDINE RESIDUE IN THE SEQUENCE-GAATTC AND FOR COUPLING SPECIFIC DNA-BINDING TO CATALYSIS [J].

JELTSCH, A ;

ALVES, J ;

OELGESCHLAGER, T ;

WOLFES, H ;

MAASS, G ;

PINGOUD, A .

JOURNAL OF MOLECULAR BIOLOGY, 1993, 229 (01) :221-234

[13] REFINEMENT OF ECO RI ENDONUCLEASE CRYSTAL-STRUCTURE - A REVISED PROTEIN CHAIN TRACING [J].

KIM, YC ;

GRABLE, JC ;

LOVE, R ;

GREENE, PJ ;

ROSENBERG, JM .

SCIENCE, 1990, 249 (4974) :1307-1309

[14]

KIM YC, 1994, STRUCTURAL BIOLOGY: THE STATE OF THE ART, VOL 1, P225

[15] CHIMERIC RESTRICTION-ENDONUCLEASE [J].

KIM, YG ;

CHANDRASEGARAN, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (03) :883-887

[16] THE ENERGETIC BASIS OF SPECIFICITY IN THE ECO RI ENDONUCLEASE DNA INTERACTION [J].

LESSER, DR ;

KURPIEWSKI, MR ;

JENJACOBSON, L .

SCIENCE, 1990, 250 (4982) :776-786

[17] STRUCTURE OF THE DNA-ECO RI ENDONUCLEASE RECOGNITION COMPLEX AT 3 A RESOLUTION [J].

MCCLARIN, JA ;

FREDERICK, CA ;

WANG, BC ;

GREENE, P ;

BOYER, HW ;

GRABLE, J ;

ROSENBERG, JM .

SCIENCE, 1986, 234 (4783) :1526-1541

[18] STRUCTURE OF BAM-HI ENDONUCLEASE BOUND TO DNA - PARTIAL FOLDING AND UNFOLDING ON DNA-BINDING [J].

NEWMAN, M ;

STRZELECKA, T ;

DORNER, LF ;

SCHILDKRAUT, I ;

AGGARWAL, AK .

SCIENCE, 1995, 269 (5224) :656-663

[19] COMBINATORIAL MUTAGENESIS OF 3 MAJOR GROOVE-CONTACTING RESIDUES OF ECORI - SINGLE AND DOUBLE AMINO-ACID REPLACEMENTS RETAINING METHYLTRANSFERASE-SENSITIVE ACTIVITIES [J].

OSUNA, J ;

FLORES, H ;

SOBERON, X .

GENE, 1991, 106 (01) :7-12

[20]

Roberts R, 1993, NUCLEASES, P35

← 1 2 3 4 →