NMR spectroscopy of RNA

被引：364

作者：

Fürtig, B ^{[1
]}

Richter, C ^{[1
]}

Wöhnert, J ^{[1
]}

Schwalbe, H ^{[1
]}

机构：

[1] Goethe Univ Frankfurt, Ctr Biomol Magnet Resonance, Inst Organ Chem & Chem Biol, D-60439 Frankfurt, Germany

来源：

CHEMBIOCHEM | 2003年 / 4卷 / 10期

关键词：

conformation analysis; isotope labeling; NMR spectroscopy; resonance assignment; RNA;

D O I：

10.1002/cbic.200300700

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

NMR spectroscopy is a powerful tool for studying proteins and nucleic acids in solution. This is illustrated by the fact that nearly half of all current RNA structures were determined by using NMR techniques. Information about the structure, dynamics, and interactions with other RNA molecules, proteins, ions, and small ligands can be obtained for RNA molecules up to 100 nucleotides. This review provides insight into the resonance assignment methods that are the first and crucial step of all NMR studies, into the determination of base-pair geometry, into the examination of local and global RNA conformation, and into the detection of interaction sites of RNA. Examples of NMR investigations of RNA are given by using several different RNA molecules to illustrate the information content obtainable by NMR spectroscopy and the applicability of NMR techniques to a wide range of biologically interesting RNA molecules.

引用

页码：936 / 962

页数：27

共 155 条

[21] Determination of three-bond 1H3′-31P couplings in nucleic acids and protein nucleic acid complexes by quantitative J correlation spectroscopy [J].