Synthesis and binding studies of the 116-mer peptide containing the double cysteine-rich motifs of protein kinase C gamma

被引：2

作者：

Fukuda, H ^{[1
]}

Irie, K

Nakahara, A

Oie, K

Ohigashi, H

Wender, PA

机构：

[1] Kyoto Univ, Grad Sch Agr, Div Appl Life Sci, Kyoto 6068502, Japan

[2] Nihon PerSept Ltd, Minato Ku, Tokyo 1060032, Japan

[3] Stanford Univ, Dept Chem, Stanford, CA 94305 USA

来源：

TETRAHEDRON LETTERS | 1998年 / 39卷 / 43期

基金：

美国国家卫生研究院;

关键词：

cysteine-rich domain; phorbol ester; protein kinase C; solid phase synthesis; tumor promoter;

D O I：

10.1016/S0040-4039(98)01768-7

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

The 116-mer peptide containing the double cysteine-rich motifs of mouse protein kinase C gamma (gamma-C1A-C1B) has been synthesized using an Fmoc-solid phase strategy with a stepwise chain elongation. The peptide was purified only by reversed phase HPLC and gave satisfactory mass data (MALDI-TOF-MS and ESI-TOF-MS). Scatchard analysis of the zinc-folded peptide revealed two binding sites of distinct affinities (K-d = 6.0 and 47.0 nM) comparable to those reported by Quest and Bell for GST-gamma-C1A-C1B fusion protein prepared by DNA recombination. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

页码：7943 / 7946

页数：4

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