Cell cycle synchronization of embryonic stem cells: Effect of serum deprivation on the differentiation of embryonic bodies in vitro

被引：41

作者：

Zhang, EM ^{[1
]}

Li, XL ^{[1
]}

Zhang, SF ^{[1
]}

Chen, LQ ^{[1
]}

Zheng, XX ^{[1
]}

机构：

[1] Zhejiang Univ, Dept Biomed Engn, Hangzhou 310027, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 333卷 / 04期

关键词：

cell synchronization; differentiation; embryonic bodies; embryonic stem cells; serum deprivation;

D O I：

10.1016/j.bbrc.2005.05.200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Research on stem-cell transplantation has indicated that the success of transplantation largely depends on synchronizing donor cells into the G0/G1 phase. In this study, we investigated the profile of embryonic stem (ES) cell synchronization and its effect on the formation of embryonic bodies (EBs) using cell culture with serum deprivation. The D3 cell line of ES cells was used, and parameters such as cell proliferation and activity, EB formation, and expression of stage-specific embryonic antigen-1 and Oct-4 were investigated. Results showed that the percentage of G0/G1 stage in serum deprivation culture is significantly higher than that in culture with serum supplementation. Synchronized ES cells can reenter the normal cell cycle successfully after serum supply. EBs formed from synchronized ES cells have higher totipotency capability to differentiate into functional neuronal cells than EBs formed from unsynchronized ES cells. Our study provides a method for ES treatment before cell transplantation that possibly helps to decrease the rate of cell death after transplantation. (c) 2005 Published by Elsevier Inc.

引用

页码：1171 / 1177

页数：7

共 18 条

[1]

Benninger Y, 2000, BRAIN PATHOL, V10, P330

[2] Embryonic stem cells develop into functional dopaminergic neurons after transplantation in a Parkinson rat model [J].

Björklund, LM ;

Sánchez-Pernaute, R ;

Chung, SM ;

Andersson, T ;

Chen, IYC ;

McNaught, KS ;

Brownell, AL ;

Jenkins, BG ;

Wahlestedt, C ;

Kim, KS ;

Isacson, O .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (04) :2344-2349

[3] In vitro synchronization of mammalian astrocytic cultures by serum deprivation [J].

Chou, RC ;

Langan, TJ .

BRAIN RESEARCH PROTOCOLS, 2003, 11 (03) :162-167

[4] IDENTIFICATION OF PROGRAMMED CELL-DEATH INSITU VIA SPECIFIC LABELING OF NUCLEAR-DNA FRAGMENTATION [J].

GAVRIELI, Y ;

SHERMAN, Y ;

BENSASSON, SA .

JOURNAL OF CELL BIOLOGY, 1992, 119 (03) :493-501

[5] Dopamine neurons derived from embryonic stem cells function in an animal model of Parkinson's disease [J].

Kim, JH ;

Auerbach, JM ;

Rodríguez-Gómez, JA ;

Velasco, I ;

Gavin, D ;

Lumelsky, N ;

Lee, SH ;

Nguyen, J ;

Sánchez-Pernaute, R ;

Bankiewicz, K ;

McKay, R .

NATURE, 2002, 418 (6893) :50-56

[6] Cell cycle synchronization of porcine fetal fibroblasts: Effects of serum deprivation and reversible cell cycle inhibitors [J].

Kues, WA ;

Anger, M ;

Carnwath, JW ;

Paul, D ;

Motlik, J ;

Niemann, H .

BIOLOGY OF REPRODUCTION, 2000, 62 (02) :412-419

[7] Embryonic stem cells differentiate into oligodendrocytes and myelinate in culture and after spinal cord transplantation [J].

Liu, S ;

Qu, Y ;

Stewart, TJ ;

Howard, MJ ;

Chakrabortty, S ;

Holekamp, TF ;

McDonald, JW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (11) :6126-6131

[8] THE PLANT AMINO-ACID MIMOSINE MAY INHIBIT INITIATION AT ORIGINS OF REPLICATION IN CHINESE-HAMSTER CELLS [J].

MOSCA, PJ ;

DIJKWEL, PA ;

HAMLIN, JL .

MOLECULAR AND CELLULAR BIOLOGY, 1992, 12 (10) :4375-4383

[9] Oct-4:: Lessons of totipotency from embryonic stem cells [J].

Pesce, M ;

Anastassiadis, K ;

Schöler, HR .

CELLS TISSUES ORGANS, 1999, 165 (3-4) :144-152

[10]

Reddy GPV, 1997, BLOOD, V90, P2293

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