Spectrophotometric determination of trifluoperazine HCl and isopropamide iodide in binary mixture using second derivative and second derivative of the ratio spectra methods

Two methods are presented for the simultaneous determination of trifluoperazine hydrochloride and isopropamide iodide in binary mixture. The first method depends on second derivative (D-2) ultraviolet spectrophotometry, with zero crossing and peak to base measurement. The second derivative amplitudes at 270.4 and 230.2 nm were selected for the assay of trifluoperazine hydrochloride and isopropamide iodide, respectively. The second method depends on second derivative of the ratio spectra by division of the absorption spectrum of the binary mixture by a normalized spectrum of one of the components and then calculating the second derivative of the ratio spectrum. The second derivative of the ratio amplitudes at 257 and 228 nm were selected for the determination of trifluoperazine hydrochloride and isopropamide iodide, respectively. The two proposed methods were successfully applied to the determination of the two drugs in laboratory prepared mixtures and in commercial tablets. (C) 2001 Elsevier Science B.V. All rights reserved.