Deposition of Yarrowia lipolytica on plasma prepared teflonlike thin films

被引：19

作者：

Lehocky, M. ^{[1
,2
]}

Amaral, P. F. F. ^{[1
,3
]}

Stahel, P. ^{[4
]}

Coelho, M. A. Z. ^{[3
]}

Barros-Timmons, A. M. ^{[1
]}

Coutinho, J. A. P. ^{[1
]}

机构：

[1] Univ Aveiro, Dept Chem, CICECO, P-3810193 Aveiro, Portugal

[2] Tomas Bata Univ Zlin, Zlin 76001, Czech Republic

[3] Univ Fed Rio de Janeiro, Sch Chem, Dept Biochem Engn, BR-21949590 Rio De Janeiro, Brazil

[4] Masaryk Univ, Fac Sci, Dept Phys Elect, CZ-61137 Brno, Czech Republic

来源：

SURFACE ENGINEERING | 2008年 / 24卷 / 01期

关键词：

Yarrowia lipolytica; plasma deposition; cell adhesion; thin films; barrier discharge; oil degradation;

D O I：

10.1179/174329408X271444

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

The adhesion of Yarrowia lipolytica to teflonlike thin films deposited by plasma on polycarbonate substrates was investigated through a series of tests in order to develop a substrate for strong and selective adhesion of Yarrowia lipolytica cells. Teflonlike thin films were prepared using atmospheric pressure surface barrier discharge with mixtures of octafluorocyclobutane (C4F8) and nitrogen as plasma gas. A variety of plasma gas feedrates and different deposition times were studied. The films were characterised by Fourier transform infrared and contact angle measurements using the sessile drop technique. Total surface energy and its components were calculated using the acid base theory. Attachment of the yeast cells was assessed by optical and scanning electron microscopy. The optimal deposition conditions for cell adhesion were determined using standard adhesion tests.

引用

页码：23 / 27

页数：5

共 16 条

[1] Cell surface characterization of Yarrowia lipolytica IMUFRJ 50682 [J].

Amaral, P. F. F. ;

Lehocky, M. ;

Barros-Timmons, A. M. V. ;

Rocha-Leao, M. H. M. ;

Coelho, M. A. Z. ;

Coutinho, J. A. P. .

YEAST, 2006, 23 (12) :867-877

[2]

Bursikova V., 2004, SURFACE ENERGY EVALU

[3] Identification and biodegradation potential of tropical aerobic hydrocarbon-degrading microorganisms [J].

Chaillan, F ;

Le Flèche, A ;

Bury, E ;

Phantavong, YH ;

Grimont, P ;

Saliot, A ;

Oudot, J .

RESEARCH IN MICROBIOLOGY, 2004, 155 (07) :587-595

[4] Selection of new over-producing derivatives for the improvement of extracellular lipase production by the non-conventional yeast Yarrowia lipolytica [J].

Fickers, P ;

Fudalej, F ;

Nicaud, JM ;

Destain, J ;

Thonart, P .

JOURNAL OF BIOTECHNOLOGY, 2005, 115 (04) :379-386

[5] Aging mechanisms of perfluorocarbon emulsions using image analysis [J].

Freire, MG ;

Dias, AMA ;

Coelho, MAZ ;

Coutinho, JAP ;

Marrucho, IM .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2005, 286 (01) :224-232

[6] Patterned assembly of Yarrowia lipolytica yeast cells onto thermally, evaporated octadecylamine films [J].

Gole, A ;

Dixit, V ;

Lala, N ;

Sainkar, SR ;

Pant, A ;

Sastry, M .

COLLOIDS AND SURFACES B-BIOINTERFACES, 2002, 25 (04) :363-368

[7]

GOODE R, 1993, COMMERCIAL AND CONSUMER LAW, P3

[8] Use of Yarrowia lipolytica strains for the treatment of olive mill wastewater [J].

Lanciotti, R ;

Gianotti, A ;

Baldi, D ;

Angrisani, R ;

Suzzi, G ;

Mastrocola, D ;

Guerzoni, ME .

BIORESOURCE TECHNOLOGY, 2005, 96 (03) :317-322

[9] Plasma surface modification of polyethylene [J].

Lehocky, M ;

Drnovská, H ;

Lapciková, B ;

Barros-Timmons, AM ;

Trindade, T ;

Zembala, M ;

Lapcik, L .

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 2003, 222 (1-3) :125-131

[10] Deposition/detachment of particles on plasma treated polymer surfaces [J].

Lehocky, M ;

Lapcik, L ;

Neves, MC ;

Trindade, T ;

Szyk-Warszynska, L ;

Warszynski, P ;

Hui, D .

THERMEC'2003, PTS 1-5, 2003, 426-4 :2533-2538

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