Patterned assembly of Yarrowia lipolytica yeast cells onto thermally, evaporated octadecylamine films

被引：9

作者：

Gole, A

Dixit, V

Lala, N

Sainkar, SR

Pant, A

Sastry, M

机构：

[1] Natl Chem Lab, Div Mat Sci, Pune 411008, Maharashtra, India

[2] Natl Chem Lab, Div Biochem Sci, Pune 411008, Maharashtra, India

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2002年 / 25卷 / 04期

关键词：

cell attachment; immobilization; lipid bilayers; quartz crystal microbalance; hydrophobic surface;

D O I：

10.1016/S0927-7765(02)00005-X

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Thermally evaporated patterned films of the lipid, octadecylamine (ODA), have been used in the immobilization of the hydrocarbon-degrading cells, Yarrowia lipolytica. The immobilization of the yeast cells occurs on hydrophobic surfaces presented by the lipid film elements in the patterned structure, the attachment of the cells to the lipid film occurring possibly through hydrophobic interactions between the hydrocarbon chains of the fatty amine film and the cell wall of the yeasts. It is observed that the cell immobilization is extremely faithful to the underlying lipid template indicating potential use in tissue engineering as well as materials applications involving specific enzyme-based biotransformations. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：363 / 368

页数：6

共 22 条

[1] Geometric control of cell life and death [J].