Polyelectrolyte multilayer microspheres as carriers for bienzyme system: Preparation and characterization

被引：31

作者：

Balabushevich, NG

Sukhorukov, GB

Larionova, NI ^{[1
]}

机构：

[1] Moscow MV Lomonosov State Univ, Dept Chem, Moscow 119992, Russia

[2] Max Planck Inst Colloids & Interfaces, D-14424 Potsdam, Germany

来源：

MACROMOLECULAR RAPID COMMUNICATIONS | 2005年 / 26卷 / 14期

关键词：

bienzyme system; melamine formaldehyde; microencapsulation; microspheres; polyelectrolytes;

D O I：

10.1002/marc.200500141

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Polyelectrolyte multilayer microspheres were prepared by alternating adsorption of dextran sulfate and protamine on melamine formaldehyde cores followed by the partial decomposition of the core. Peroxidase and glucose oxidase were immobilized in the prepared microspheres. Retention of enzymatic activity of the peroxidase/glucose oxidase system incorporated into the microspheres was demonstrated, Applicability of the bienzyme system immobilized in the microspheres for kinetic glucose assay was shown.

引用

收藏

页码：1168 / 1172

页数：5

相关论文

共 31 条

[1] Biomedical applications of electrostatic layer-by-layer nano-assembly of polymers, enzymes, and nanoparticles [J].

Ai, H ;

Jones, SA ;

Lvov, YM .

CELL BIOCHEMISTRY AND BIOPHYSICS, 2003, 39 (01) :23-43

[2] Loading the multilayer dextran sulfate/protamine microsized capsules with peroxidase [J].

Balabushevich, NG ;

Tiourina, OP ;

Volodkin, DV ;

Larionova, NI ;

Sukhorukov, GB .

BIOMACROMOLECULES, 2003, 4 (05) :1191-1197

[3] Fabrication and characterization of polyelectrolyte microparticles with protein [J].

Balabushevich, NG ;

Larionova, NI .

BIOCHEMISTRY-MOSCOW, 2004, 69 (07) :757-762

[4] Encapsulation of catalase in polyelectrolyte microspheres composed of melamine formaldehyde, dextran sulfate, and protamine [J].

Balabushevich, NG ;

Zimina, EP ;

Larioniva, NI .

BIOCHEMISTRY-MOSCOW, 2004, 69 (07) :763-769

[5] Encapsulation of proteins by layer-by-layer adsorption of polyelectrolytes onto protein aggregates: Factors regulating the protein release [J].

Balabushevitch, NG ;

Sukhorukov, GB ;

Moroz, NA ;

Volodkin, DV ;

Larionova, NI ;

Donath, E ;

Mohwald, H .

BIOTECHNOLOGY AND BIOENGINEERING, 2001, 76 (03) :207-213

[6] Enzyme multilayers on colloid particles:: Assembly, stability, and enzymatic activity [J].

Caruso, F ;

Schüler, C .

LANGMUIR, 2000, 16 (24) :9595-9603

[7] Assembly of β-glucosidase multilayers on spherical colloidal particles and their use as active catalysts [J].

Caruso, F ;

Fiedler, H ;

Haage, K .

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 2000, 169 (1-3) :287-293

[8] Enzyme encapsulation in layer-by-layer engineered polymer multilayer capsules [J].

Caruso, F ;

Trau, D ;

Möhwald, H ;

Renneberg, R .

LANGMUIR, 2000, 16 (04) :1485-1488

[9] Two future generations of blood substitutes based on polyhemoglobin-SOD-catalase and nanoencapsulation [J].

Chang, TMS ;

D'Agnillo, F ;

Yu, WP ;

Razack, S .

ADVANCED DRUG DELIVERY REVIEWS, 2000, 40 (03) :213-218

[10] Novel formulations of vitamins and insulin by nanoengineering of polyelectrolyte multilayers around microcrystals [J].

Dai, ZF ;

Heilig, A ;

Zastrow, H ;

Donath, E ;

Möhwald, H .

CHEMISTRY-A EUROPEAN JOURNAL, 2004, 10 (24) :6369-6374

← 1 2 3 4 →