Polyelectrolyte multilayer microspheres as carriers for bienzyme system: Preparation and characterization

被引：31

作者：

Balabushevich, NG

Sukhorukov, GB

Larionova, NI ^{[1
]}

机构：

[1] Moscow MV Lomonosov State Univ, Dept Chem, Moscow 119992, Russia

[2] Max Planck Inst Colloids & Interfaces, D-14424 Potsdam, Germany

来源：

MACROMOLECULAR RAPID COMMUNICATIONS | 2005年 / 26卷 / 14期

关键词：

bienzyme system; melamine formaldehyde; microencapsulation; microspheres; polyelectrolytes;

D O I：

10.1002/marc.200500141

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Polyelectrolyte multilayer microspheres were prepared by alternating adsorption of dextran sulfate and protamine on melamine formaldehyde cores followed by the partial decomposition of the core. Peroxidase and glucose oxidase were immobilized in the prepared microspheres. Retention of enzymatic activity of the peroxidase/glucose oxidase system incorporated into the microspheres was demonstrated, Applicability of the bienzyme system immobilized in the microspheres for kinetic glucose assay was shown.

引用

页码：1168 / 1172

页数：5

共 31 条

[21] Design and characterization of immobilized enzymes in microfluidic systems [J].

Mao, HB ;

Yang, TL ;

Cremer, PS .

ANALYTICAL CHEMISTRY, 2002, 74 (02) :379-385

[22]

PAVLENKO IM, 2002, RUSS J BIOORG CHEM+, V1, P50

[23] Tailor-made polyelectrolyte microcapsules:: From multilayers to smart containers [J].

Peyratout, CS ;

Dähne, L .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2004, 43 (29) :3762-3783

[24]

Schüler C, 2000, MACROMOL RAPID COMM, V21, P750, DOI 10.1002/1521-3927(20000701)21:11<750::AID-MARC750>3.0.CO

[25]

2-3

[26]

Tiourina OP, 2001, MACROMOL BIOSCI, V1, P209, DOI 10.1002/1616-5195(20010701)1:5<209::AID-MABI209>3.0.CO

[27]

2-Y

[28] Encapsulation of glucose oxidase microparticles within a nanoscale layer-by-layer film: immobilization and biosensor applications [J].

Trau, D ;

Renneberg, R .

BIOSENSORS & BIOELECTRONICS, 2003, 18 (12) :1491-1499

[29]

Volodkin DV, 2003, STP PHARMA SCI, V13, P163

[30] Microfabricated protein-containing poly(ethylene glycol) hydrogel arrays for biosensing [J].

Yadavalli, VK ;

Koh, WG ;

Lazur, GJ ;

Pishko, MV .

SENSORS AND ACTUATORS B-CHEMICAL, 2004, 97 (2-3) :290-297

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