Internal pH indicators for biomolecular NMR

被引：71

作者：

Baryshnikova, Olga K. ^{[1
]}

Williams, Thomas C. ^{[1
]}

Sykes, Brian D. ^{[1
]}

机构：

[1] Univ Alberta, Dept Biochem, Edmonton, AB T6G 2H7, Canada

来源：

JOURNAL OF BIOMOLECULAR NMR | 2008年 / 41卷 / 01期

关键词：

internal indicator; biomolecular NMR; pH;

D O I：

10.1007/s10858-008-9234-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a non-invasive technique for determining pH in biomolecular NMR sample using buffer components (formate, tris, piperazine, and imidazole) as internal pH indicators, whose H-1 NMR chemical shifts are sensitive to pH in a range from 2.5 to 9.8. This method is suitable for a wide range of applications where samples are handled intensively during NMR titrations or in high throughput analysis in structural genomics or metabolomics.

引用

页码：5 / 7

页数：3

共 12 条

[1]

DEFRONZO M, 1987, J BIOL CHEM, V262, P11032

[2] Binding of cardiac troponin-I147-163 induces a structural opening in human cardiac tropoin-C [J].

Li, MX ;

Spyracopoulos, L ;

Sykes, BD .

BIOCHEMISTRY, 1999, 38 (26) :8289-8298

[3] Structure and dynamics of the C-domain of human cardiac troponin C in complex with the inhibitory region of human cardiac troponin I [J].

Lindhout, DA ;

Sykes, BD .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (29) :27024-27034

[4] α- and β-phosphorylated amines and pyrrolidines, a new class of low toxic highly sensitive 31P NMR pH indicators -: Modeling of pKα and chemical shift values as a function of substituents [J].

Pietri, S ;

Miollan, M ;

Martel, S ;

Le Moigne, F ;

Blaive, B ;

Culcasi, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (26) :19505-19512

[5]

Pietropaolo M, 2001, Pediatr Diabetes, V2, P2

[6] PROTON NUCLEAR-MAGNETIC-RESONANCE SPECTROSCOPY OF AQUEOUS-SOLUTIONS - COMPLETE ELIMINATION OF THE WATER RESONANCE BY SPIN SPIN RELAXATION [J].

RABENSTEIN, DL ;

FAN, S .

ANALYTICAL CHEMISTRY, 1986, 58 (14) :3178-3184

[7] Structure of cardiac muscle troponin C unexpectedly reveals a closed regulatory domain [J].

Sia, SK ;

Li, MX ;

Spyracopoulos, L ;

Gagne, SM ;

Liu, W ;

Putkey, JA ;

Sykes, BD .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (29) :18216-18221

[8] Spectroscopic measurement of a long-predicted active site pK in iron-superoxide dismutase from Escherichia coli [J].

Sorkin, DL ;

Miller, AF .

BIOCHEMISTRY, 1997, 36 (16) :4916-4924

[9] 1H/31P NMR pH indicator series to eliminate the glass electrode in NMR spectroscopic pKa determinations [J].

Szakács, Z ;

Hägele, G ;

Tyka, R .

ANALYTICA CHIMICA ACTA, 2004, 522 (02) :247-258

[10] F-19-NUCLEAR MAGNETIC-RESONANCE - MEASUREMENTS OF [O-2] AND PH IN BIOLOGICAL-SYSTEMS [J].

TAYLOR, J ;

DEUTSCH, C .

BIOPHYSICAL JOURNAL, 1988, 53 (02) :227-233

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