Increased expression of dendritic mRNA following the induction of long-term potentiation

被引:64
作者
Roberts, LA
Large, CH
Higgins, MJ
Stone, TW
O'Shaughnessy, CT
Morris, BJ
机构
[1] Univ Glasgow, Inst Biomed & Life Sci, Div Neurosci & Biomed Syst, Pharmacol Labs, Glasgow, Lanark, Scotland
[2] Glaxo Wellcome Res & Dev Ltd, Med Res Ctr, Stevenage SG1 2NY, Herts, England
来源
MOLECULAR BRAIN RESEARCH | 1998年 / 56卷 / 1-2期
基金
英国惠康基金;
关键词
calcium-calmodulin-dependent protein kinase II; microtubule-associated protein 2; long-term potentiation; rat; gene expression;
D O I
10.1016/S0169-328X(98)00026-6
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
A small number of mRNAs, including Ca2+/calmodulin-dependent protein kinase II cr-subunit (CamKII alpha) mRNA and microtubule-associated protein 2 (MAP2) mRNA, are present in the dendrites of neurones as well as in the cell bodies. We show here that the induction of long-term potentiation (LTP) in the hippocampal perforant path/granule cell synapses in anaesthetised rats is associated with increased levels of CamKII alpha mRNA and MAP;! mRNA in the granule cell dendrites after 2 h. Similarly, induction of LTP in the Schaffer collateral/CAl pyramidal cell synapses in hippocampal slices maintained in vitro also results in elevated dendritic levels of CamKII alpha: mRNA and MAP;! mRNA 2 h later. In both models, the levels of various other mRNA species restricted to the cell body region were unaffected by the induction of LTP. Increased expression of dendritic CamKII alpha mRNA and MAP2 mRNA appears to be a general feature of hippocampal plasticity, since it occurs following LTP induction in both the dentate gyrus and the CA1 region. The elevation of mRNA levels in a restricted region close to the afferent synapses would allow a highly-localised enhancement of the synthesis of the corresponding proteins, providing an elegant mechanism for protein-synthesis-dependent synaptic plasticity to maintain a high degree of anatomical specificity. (C) 1998 Elsevier Science B.V.
引用
收藏
页码:38 / 44
页数:7
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