Bone marrow cell-seeded biodegradable polymeric scaffold enhances angiogenesis and improves function of the infarcted heart

被引:24
作者
Fukuhara, S
Tomita, S
Nakatani, T
Fujisato, T
Ohtsu, Y
Ishida, M
Yutani, C
Kitamura, O
机构
[1] Natl Cardiovasc Ctr, Dept Regenerat Med & Tissue Engn, Suita, Osaka 5658565, Japan
[2] Natl Cardiovasc Ctr, Dept Pathol, Suita, Osaka 5658565, Japan
[3] Natl Cardiovasc Ctr, Dept Cardiovasc Surg, Suita, Osaka 5658565, Japan
[4] Natl Cardiovasc Ctr, Dept Organ Transplantat, Suita, Osaka 5658565, Japan
关键词
angiogenesis; biomaterial; bone marrow cells; ischemic heart disease;
D O I
10.1253/circj.69.850
中图分类号
R5 [内科学];
学科分类号
1002 [临床医学]; 100201 [内科学];
摘要
Background The present study examined whether a bioengineered polyglycolic acid cloth (PGAC) impregnated with bone marrow cells (BMC) improved the function and angiogenesis of the infarcted heart. Methods and Results The coronary artery was ligated in Lewis rats and the infarcted area was covered with a PGAC in group 1 (n=8), with a PGAC containing basic-fibroblast growth factor (b-FGF) in group 2 (n=11) and a PGAC containing b-FGF and freshly isolated BMC in group 3 (n=10). In addition, BMC derived from transgenic mice expressing green fluorescent protein (GFP)-BMC were seeded into a PGAC, which was sutured over the infarcted area of C57BL/6 mice (n=5). In the rat study, developed and systolic pressures, dp/dt max and dp/dt min) were the highest in group 3, as were the capillary density in the PGAC and infarcted area. In the mouse study, there were few GFP-BMC in the PGAC, but none in the infarcted area. Conclusions A PGAC with BMC improved cardiac function by inducing angiogenesis without migration of BMC. Freshly isolated BMC work as angiogenic inducers and a PGAC is useful as a "drug delivery system".
引用
收藏
页码:850 / 857
页数:8
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