Enzymatic transformation of cephalosporin C to 7-ACA by simultaneous action of immobilized d-amino acid oxidase and glutaryl-7-ACA acylase

被引：13

作者：

Bianchi, D ^{[1
]}

Bortolo, R ^{[1
]}

Golini, P ^{[1
]}

Cesti, P ^{[1
]}

机构：

[1] EniChem SPA, Ctr Ric Novara, Ist G Donegani, I-28100 Novara, Italy

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1998年 / 73卷 / 2-3期

关键词：

enzymatic transformation; cephalosporin C; 7-amino-cephalosporanic acid; d-amino acid oxidase; glutaryl-7-ACA acylase;

D O I：

10.1007/BF02785660

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The enzymatic transformation of cephalosporin C (CEPH C) to 7-amino-cephalosporanic acid (7-ACA) using D-amino acid oxidase (DAO) and glutaryl-7-ACA acylase (Gl-7-ACA) is reported. The enzymes have been immobilized separately on different carriers, in order to maximize the catalytic activity and the stability. The reaction has been carried out in single-step-like conditions, using the two enzymes simultaneously The effect of catalase contamination, present in the DAO preparations, was balanced by addition of extra hydrogen peroxide. In optimum conditions, the conversion of CEPH C to 7-ACA was higher than 90%, with byproduct formation lower than 4%. The mixture of immobilized enzymes was reused in repeated reaction cycles, showing an appreciable operational stability.

引用

页码：257 / 268

页数：12

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