This work estimated the porosities of activated sludge flocs, cationic polyelectrolyte flocculated, based on free-settling tests, buoyant weight measurements, and confocal laser scanning microscope (CLSM) tests. The extent of advective flow was estimated based on bubble-tracking test. The former two measurements suggested a close-to-unity porosity, that is, an extremely void floe interior. Meanwhile, the latter two tests recommended a dense floe interior with a porosity less than 64%. A discrepancy exists between the porosities estimated by various tests. A floe model was proposed based on the understanding that a vast amount of bound water in the floe was regarded as void in buoyant weight measurement, but was impermeable for advective flow. The distribution rather than the mean value of the porosity controls the advective flow. There existed no simple correlation between the porosities measured by different tests. (C) 2003 Elsevier Inc. All rights reserved.