Screening and isolating potential α-glucosidase inhibitors from Rhizoma Coptidis by ultrafiltration LC-PDA-ESI/MS combined with high-speed countercurrent chromatography and reverse-phase medium-pressure liquid chromatography

被引：20

作者：

Tang, Ying ^{[1
]}

Li, Senlin ^{[1
]}

Li, Sainan ^{[1
]}

Yang, Xiaojing ^{[1
]}

Qin, Yao ^{[1
]}

Liu, Chunming ^{[1
]}

Zhang, Yuchi ^{[1
]}

机构：

[1] Changchun Normal Univ, Cent Lab, 677 North Changji Rd, Changchun 130032, Jilin, Peoples R China

来源：

MEDICINAL CHEMISTRY RESEARCH | 2017年 / 26卷 / 12期

基金：

中国国家自然科学基金;

关键词：

Rhizoma Coptidis; alpha-glucosidase inhibitors; RP-MPLC; HSCCC; Ultrafiltration LC-PDA-ESI/MS; TANDEM MASS-SPECTROMETRY; PROTOBERBERINE ALKALOIDS; SEPARATION; IDENTIFICATION; FLAVONOIDS; PLASMA; COUPLE; MS;

D O I：

10.1007/s00044-017-2031-6

中图分类号：

R914 [药物化学];

学科分类号：

100705 [微生物与生化药学];

摘要：

Rhizoma Coptidis is an alkaloid-rich herbal drug used in Chinese herbal medicine. Currently, conventional methods for screening and isolating alkaloids are labor-intensive and time-consuming. In the present study, ultrafiltration liquid chromatography with photodiode array detection coupled to electrospray ionization tandem mass spectrometry (ultrafiltration liquid chromatography-photodiode array detector-electrospary ionization mass spectrometry (LC-PDA-ESI/MS)) were applied to screen and identify alpha-glucosidase inhibitors in R. Coptidis. High-speed countercurrent chromatography and reverse-phase medium-pressure liquid chromatography were applied to separate and isolate the active constituents. As a result, five major compounds in R. Coptidis were screened and identified as alpha-glucosidase inhibitors by ultrafiltration LC-PDA-ESI/MS. Five ligands, jatrorrhizine, epiberberine, coptisine, palmatine, and berberine, were isolated by reverse-phase medium-pressure liquid chromatography and high-speed countercurrent chromatography. The purities of the five compounds as determined by high-performance liquid chromatography were 76.20, 75.13, 82.24, 93.78, and 92.01%, respectively. The results indicate that systematic isolation of bioactive components in R. Coptidis guided by ultrafiltration LC-PDA-ESI/MS is a feasible and efficient technique that could be extended to separation of other enzyme inhibitors.

引用

页码：3384 / 3394

页数：11

共 21 条

[1]

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