Genetic characterization of complex inter-recombinant HIV-1 strains circulating in Spain and reliability of distinct rapid subtyping tools

被引：14

作者：

Holguin, Africa ^{[1
]}

Lospitao, Eva ^{[1
]}

Lopez, Marisa ^{[1
]}

de Arellano, Eva Ramirez ^{[1
]}

Pena, Maria Jose ^{[2
]}

del Romero, Jorge ^{[3
]}

Martin, Cristina ^{[1
]}

Soriano, Vincent ^{[1
]}

机构：

[1] Hosp Carlos 3, Dept Infect Dis, Madrid 28029, Spain

[2] Hosp Univ Gran Canaria Dr Negrin, Microbiol Serv, Las Palmas Gran Canaria, Canary Isl, Spain

[3] Ctr Sanitario Sandoval, Madrid, Spain

来源：

JOURNAL OF MEDICAL VIROLOGY | 2008年 / 80卷 / 03期

关键词：

full-length genome; HIV-1; subtypes; recombination; CRF; subtyping tools;

D O I：

10.1002/jmv.21105

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Genetic recombination and high rate of mutation increase HIV-1 diversity, allowing viruses to escape more easily from the host immune response or antiretroviral drugs. The recombinant nature of full-length HIV-1 genomic sequences derived from viruses infecting five epidemiologically unlinked individuals carrying HIV-1 non-B variants was investigated. Overlapping PCR amplifications followed by direct sequencing of viral products derived from plasma and phylogenetic analyses were carried out. Four viral sequences clustered with CRF06_cpx and one with CRF02_AG. However, subtyping of separate genes within the same genome revealed that four were recombinant forms involving different subtypes and/or CRFs with distinct breakpoints. Two specimens included CRF02_AG and CRF06_cpx sequences with several fragments from other HIV-1 clades along their genomes. Three rapid subtyping tools (Stanford, NCBI, and REGA) showed discrepant results when interpreting these viral sequences. This is the first description of CRF02_AG/CRF06_cpx recombinants in Spain. The results highlight the tremendous heterogeneity of HIV-1 recombinant strains currently in circulation.

引用

页码：383 / 391

页数：9

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