Hyperploidy of embryonic fibroblasts derived from Parp-1 knockout mouse

被引：10

作者：

Nozaki, T

Fujihara, H

Kamada, N

Ueda, O

Takato, T

Nakagama, H

Sugimura, T

Suzuki, H

Masutani, M

机构：

[1] Natl Canc Ctr Res Inst, Biochem Div, Chuo Ku, Tokyo 1040045, Japan

[2] Univ Tokyo, Fac Med, Dept Oral & Maxillofacial Surg, Bunkyo Ku, Tokyo 1130032, Japan

[3] Chugai Pharmaceut Co Ltd, Shizuoka 4120038, Japan

来源：

PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES | 2001年 / 77卷 / 06期

关键词：

Parp; ploidy; endoreduplication; embryonic fibroblast; genomic stability;

D O I：

10.2183/pjab.77.121

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Various studies show that poly (ADP-ribose) polymerase-1 (Parp-1) is involved in genomic stability. We previously established Parp-1 knockout (Parp-1(-/-)) mice by disrupting Parp-1 exon 1. Parp-1(-/-) and Parp-1(+/+) embryonic fibroblasts were isolated and they became immortalized spontaneously after two months of culture. Parp-1(-/-) embryonic fibroblasts showed extensive hyperploidy compared to Parp-1(+/+) counterparts, and most of the cells contained a single nucleus. These results suggest that disruption of Parp-1 could result in the perturbation of chromosomal ploidy control through endoreduplication.

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收藏

页码：121 / 124

页数：4

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