The impact of cell wall carbohydrate composition on the chitosan flocculation of Chlorella

被引：103

作者：

Cheng, Yu-Shen ^{[1
]}

Zheng, Yi ^{[1
]}

Labavitch, John M. ^{[2
]}

VanderGheynst, Jean S. ^{[1
]}

机构：

[1] Univ Calif Davis, Dept Biol & Agr Engn, Davis, CA 95616 USA

[2] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA

来源：

PROCESS BIOCHEMISTRY | 2011年 / 46卷 / 10期

关键词：

Microalgae; Flocculation; Cell wall; Carbohydrate; Chitosan; Flocculant; PARAMECIUM-BURSARIA; SUGAR COMPOSITION; NITROGEN-STARVATION; MARINE MICROALGAE; PROCESS OPTIONS; ALGAE; STRAINS; SUSPENSIONS; THROUGHPUT; BIODIESEL;

D O I：

10.1016/j.procbio.2011.06.021

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The carbohydrate composition of the algal cell wall was investigated for its role in cell flocculation. Cultures of Chlorella variabilis NC64A, which were found to have different levels of neutral sugar, uronic acid and amino sugar in the cell wall when cultured in different nitrogen sources and concentrations, were subjected to flocculation with chitosan at dosages of 0-69.6 mg/l and pH values of 5.5, 7 and 8.5. In addition, flocculations of another three strains of Chlorella, which have different levels of cell wall components, were tested. Flocculation improved for all strains at pH 8.5 suggesting that inter molecular forces such as hydrogen bonding might be more important than charge neutralization in the flocculation of Chlorella. Total carbohydrate content in the cell wall was the most significant factor positively affecting the flocculation efficiency of C. variabilis NC64A cells with different cell wall compositions and the other Chlorella strains. The results presented in this study suggest that chitosan flocculation can be improved by optimizing the cell culture conditions to achieve higher cell wall polysaccharide content or selecting an algal strain with higher cell wall polysaccharide content. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：1927 / 1933

页数：7

共 48 条

[31] HARVESTING MARINE MICROALGAE SPECIES BY CHITOSAN FLOCCULATION [J].

MORALES, J ;

DELANOUE, J ;

PICARD, G .

AQUACULTURAL ENGINEERING, 1985, 4 (04) :257-270

[32] Chitosan structure in aqueous solution [J].

Pedroni, VI ;

Schulz, PC ;

Gschaider, ME ;

Andreucetti, N .

COLLOID AND POLYMER SCIENCE, 2003, 282 (01) :100-102

[33]

Raja R, 2008, CRIT REV MICROBIOL, V34, P77, DOI [10.1080/10408410802086783, 10.1080/10408410802086783 ]

[34]

REISSER W, 1988, SYMBIOSIS, V6, P253

[35] Chitosan for coagulation/flocculation processes - An eco-friendly approach [J].

Renault, F. ;

Sancey, B. ;

Badot, P. -M. ;

Crini, G. .

EUROPEAN POLYMER JOURNAL, 2009, 45 (05) :1337-1348

[36] EFFECTS OF NITROGEN STARVATION ON THE FUNCTION AND ORGANIZATION OF THE PHOTOSYNTHETIC MEMBRANES IN CRYPTOMONAS-MACULATA (CRYPTOPHYCEAE) [J].

RHIEL, E ;

KRUPINSKA, K ;

WEHRMEYER, W .

PLANTA, 1986, 169 (03) :361-369

[37] The use of chitosan as a flocculant in mammalian cell culture dramatically improves clarification throughput without adversely impacting monoclonal antibody recovery [J].

Riske, Frank ;

Schroeder, James ;

Belliveau, Julie ;

Kang, Xuezhen ;

Kutzko, Joseph ;

Menon, Manoj K. .

JOURNAL OF BIOTECHNOLOGY, 2007, 128 (04) :813-823

[38] Second Generation Biofuels: High-Efficiency Microalgae for Biodiesel Production [J].

Schenk, Peer M. ;

Thomas-Hall, Skye R. ;

Stephens, Evan ;

Marx, Ute C. ;

Mussgnug, Jan H. ;

Posten, Clemens ;

Kruse, Olaf ;

Hankamer, Ben .

BIOENERGY RESEARCH, 2008, 1 (01) :20-43

[39] Commercial applications of microalgae [J].

Spolaore, P ;

Joannis-Cassan, C ;

Duran, E ;

Isambert, A .

JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2006, 101 (02) :87-96

[40] Interactions between chitosans and bacterial suspensions:: adsorption and flocculation [J].

Strand, SP ;

Vårum, KM ;

Ostgaard, K .

COLLOIDS AND SURFACES B-BIOINTERFACES, 2003, 27 (01) :71-81

← 1 2 3 4 5 →