Topors acts as a SUMO-1 E3 ligase for p53 in vitro and in vivo

被引：130

作者：

Weger, S ^{[1
]}

Hammer, E ^{[1
]}

Heilbronn, R ^{[1
]}

机构：

[1] Free Univ Berlin, Inst Infect Dis, Dept Virol, D-12203 Berlin, Germany

来源：

FEBS LETTERS | 2005年 / 579卷 / 22期

关键词：

small ubiquitin-like modifier; Topors; p53; E3; ligase; RING finger;

D O I：

10.1016/j.febslet.2005.07.088

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human Topors, which was originally identified as cellular binding partner of DNA topoisomerase I and of p53, has recently been shown to function as an ubiquitin E3 ligase for p53 in a manner dependent on its N'-terminally located RING finger. Here, we demonstrate that Topors also enhances the conjugation of the small ubiquitin-like modifier 1 (SUMO-1) to p53 in vivo and in a reconstituted in vitro system. The Topors SUMO-1 E3 ligase activity does not depend upon its RING finger motif. In HeLa cells, Topors induced p53 sumoylation was accompanied by an increase in endogenous p53 protein levels. Furthermore, Topors enhances the sumoylation of a variety of other, yet unidentified, cellular proteins. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：5007 / 5012

页数：6

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