Enhanced surface plasmon resonance immunosensing using a streptavidin-biotinylated protein complex

被引：27

作者：

Pei, RJ

Yang, XR

Wang, EK ^{[1
]}

机构：

[1] Chinese Acad Sci, Changchun Inst Appl Chem, Electroanalyt Chem Lab, Changchun 130022, Jilin, Peoples R China

[2] Chinese Acad Sci, Changchun Inst Appl Chem, Natl Analyt Res Ctr Electrochem & Spect, Changchun 130022, Jilin, Peoples R China

来源：

ANALYST | 2001年 / 126卷 / 01期

关键词：

D O I：

10.1039/b007432m

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this paper, we present a novel strategy for improving the sensitivity of surface plasmon resonance immunosensing using a streptavidin-biotinylated protein complex. This amplification strategy is based on the construction of a molecular complex between streptavidin and biotin labeled protein. The complex can be formed in a cross-linking network of molecules so that the amplification of the response signal will be realized due to the big molecular size of the complex. The results show that the amplification strategy causes a dramatic improvement of the detection sensitivity. hIgG protein could be detected in the range of 0.005-10 mug ml(-1).

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页码：4 / 6

页数：3

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