Bacterial response to eukaryotic cells analysis of differentially expressed proteins using nano liquid chromatography-electrospray ionization tandem mass spectrometry

被引:4
作者
Alpert, C
Engst, W
Guehler, A
Oelschlaeger, T
Blaut, M
机构
[1] German Inst Human Nutr Potsdam Rehbruecke, Dept Gastrointestinal Microbiol, D-14558 Nuthetal, Germany
[2] Univ Wurzburg, D-97070 Wurzburg, Germany
关键词
host-bacteria interaction; proteomics; NanoLC-MS/MS;
D O I
10.1016/j.chroma.2005.03.094
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Host-bacteria interactions have mostly been investigated with regard to the host response or to activities of pathogenic bacteria. In contrast, we aim to identify reactions of non-pathogenic bacteria that result from their contact with host cells of the gastrointestinal tract. In a proteomic approach, the response of non-pathogenic human Escherichia coli bacteria on gut epithelial cells (rat IEC-6) was investigated in an in vitro co-culture model. For this purpose, a sensitive analytical procedure was developed based on the identification of two-dimensional polyacrylamide gel electrophoresis separated proteins by online nanoLC-electrospray ionization MS/MS using a quadrupole time-of-flight tandem mass spectrometer for accurate mass determination. We demonstrate here the efficiency of this technique by the identification of a total of 43 differentially expressed proteins, out of which 25 were up-regulated and 18 were down-regulated. They represent a wide range of molecular weight and different metabolic and physiological functions. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:25 / 32
页数:8
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