Proteomic LC-MS systems using nanoscale liquid chromatography with tandem mass spectrometry

被引：134

作者：

Ishihama, Y ^{[1
]}

机构：

[1] Eisai & Co Ltd, Lab Seeds Finding Technol, Tsukuba, Ibaraki 3002635, Japan

来源：

JOURNAL OF CHROMATOGRAPHY A | 2005年 / 1067卷 / 1-2期

关键词：

proteome; proteomics; NanoLC-MS/MS;

D O I：

10.1016/j.chroma.2004.10.107

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Current nano-scale liquid chromatography-tandem mass spectrometry (nanoLC-MS/MS) approaches in proteome research are reviewed from an analytical perspective. For comprehensive analysis of cellular proteins, analytical methods with higher resolution, sensitivity, and wider dynamic range are required. Miniaturized LC coupled with tandem mass spectrometry is currently one of the most versatile techniques. In this review, the current status of nanoLC-MS/MS systems as well as data management systems is addressed. In addition, the future prospects for complete proteomics are discussed. (c) 2004 Elsevier B.V. All rights reserved.

引用

页码：73 / 83

页数：11

共 111 条

[1] Mass spectrometry-based proteomics [J].

Aebersold, R ;

Mann, M .

NATURE, 2003, 422 (6928) :198-207

[2] The human plasma proteome - History, character, and diagnostic prospects [J].

Anderson, NL ;

Anderson, NG .

MOLECULAR & CELLULAR PROTEOMICS, 2002, 1 (11) :845-867

[3] The human plasma proteome - A nonredundant list developed by combination of four separate sources [J].

Anderson, NL ;

Polanski, M ;

Pieper, R ;

Gatlin, T ;

Tirumalai, RS ;

Conrads, TP ;

Veenstra, TD ;

Adkins, JN ;

Pounds, JG ;

Fagan, R ;

Lobley, A .

MOLECULAR & CELLULAR PROTEOMICS, 2004, 3 (04) :311-326

[4] A design for low-flow sheathless electrospray emitters [J].

Barnidge, DR ;

Nilsson, S ;

Markides, KE .

ANALYTICAL CHEMISTRY, 1999, 71 (19) :4115-4118

[5]

BENEVIDES CC, 2003, P 51 ASMS C MASS SPE, DOI UNSP TPP294

[6] A proteomics strategy to elucidate functional protein-protein interactions applied to EGF signaling [J].

Blagoev, B ;

Kratchmarova, I ;

Ong, SE ;

Nielsen, M ;

Foster, LJ ;

Mann, M .

NATURE BIOTECHNOLOGY, 2003, 21 (03) :315-318

[7] MICROBORE HPLC-MASS SPECTROMETRY FOR THE ANALYSIS OF PEPTIDE MIXTURES USING A CONTINUOUS-FLOW INTERFACE [J].

CAPRIOLI, RM ;

DAGUE, B ;

FAN, T ;

MOORE, WT .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1987, 146 (01) :291-299

[8] Immobilized pH gradients as a first dimension in shotgun proteomics and analysis of the accuracy of pI predictability of peptides [J].

Cargile, BJ ;

Talley, DL ;

Stephenson, JL .

ELECTROPHORESIS, 2004, 25 (06) :936-945

[9] POROUS CERAMIC BED SUPPORTS FOR FUSED-SILICA PACKED CAPILLARY COLUMNS USED IN LIQUID-CHROMATOGRAPHY [J].

CORTES, HJ ;

PFEIFFER, CD ;

RICHTER, BE ;

STEVENS, TS .

JOURNAL OF HIGH RESOLUTION CHROMATOGRAPHY & CHROMATOGRAPHY COMMUNICATIONS, 1987, 10 (08) :446-448

[10] De novo peptide sequencing via tandem mass spectrometry [J].

Dancík, V ;

Addona, TA ;

Clauser, KR ;

Vath, JE ;

Pevzner, PA .

JOURNAL OF COMPUTATIONAL BIOLOGY, 1999, 6 (3-4) :327-342

← 1 2 3 4 5 6 7 8 9 10 →