Genomic structure of the bovine PrP gene and complete nucleotide sequence of bovine PrP cDNA

被引：21

作者：

Horiuchi, M

Ishiguro, N

Nagasawa, H

Toyoda, Y

Shinagawa, M

机构：

[1] Obihiro Univ Agr & Vet Med, Dept Vet Publ Hlth, Obihiro, Hokkaido 080, Japan

[2] Obihiro Univ Agr & Vet Med, Inst Protozoan Mol Immunol, Obihiro, Hokkaido 080, Japan

来源：

ANIMAL GENETICS | 1998年 / 29卷 / 01期

关键词：

BSE; long-PCR; 3 '-UTR; prion protein; scrapie;

D O I：

10.1046/j.1365-2052.1998.00250.x

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The present authors previously reported the nucleotide sequence of the 5' half of a cDNA encoding bovine prion protein (PrP) and the genomic structure of the bovine PrP gene encoding the 5'-untranslated region. Here they report the extent of intron 2 of the bovine PrP gene and the nucleotide sequence of the 3' half of bovine PrP cDNA that had not been determined before. This newly sequenced 3' half of the bovine PrP cDNA consisted of 2149 bp. The entire 3'-untranslated region (3'-UTR) was found to be encoded by a single exon, exon 3. One nucleotide polymorphism was found in the 3'-UTR. The length of intron 2 was estimated to be about 14 kbp. The structure of bovine PrP gene can be defined by combining the present results and previous reports on the bovine PrP gene.

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页码：37 / 40

页数：4

相关论文

共 11 条

[1] OLIGODEOXYRIBONUCLEOTIDE LIGATION TO SINGLE-STRANDED CDNAS - A NEW TOOL FOR CLONING 5'-ENDS OF MESSENGER-RNAS AND FOR CONSTRUCTING CDNA LIBRARIES BY INVITRO AMPLIFICATION [J].

EDWARDS, JBDM ;

DELORT, J ;

MALLET, J .

NUCLEIC ACIDS RESEARCH, 1991, 19 (19) :5227-5232

[2] 2 ALLELES OF A NEURAL PROTEIN GENE LINKED TO SCRAPIE IN SHEEP [J].

GOLDMANN, W ;

HUNTER, N ;

FOSTER, JD ;

SALBAUM, JM ;

BEYREUTHER, K ;

HOPE, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (07) :2476-2480

[3] DIFFERENT FORMS OF THE BOVINE PRP GENE HAVE 5 OR 6 COPIES OF A SHORT, G-C-RICH ELEMENT WITHIN THE PROTEIN-CODING EXON [J].

GOLDMANN, W ;

HUNTER, N ;

MARTIN, T ;

DAWSON, M ;

HOPE, J .

JOURNAL OF GENERAL VIROLOGY, 1991, 72 :201-204

[4] PRP GENOTYPE AND AGENT EFFECTS IN SCRAPIE - CHANGE IN ALLELIC INTERACTION WITH DIFFERENT ISOLATES OF AGENT IN SHEEP, A NATURAL HOST OF SCRAPIE [J].

GOLDMANN, W ;

HUNTER, N ;

SMITH, G ;

FOSTER, J ;

HOPE, J .

JOURNAL OF GENERAL VIROLOGY, 1994, 75 :989-995

[5] Alternative usage of exon 1 of bovine PrP mRNA [J].

Horiuchi, M ;

Ishiguro, N ;

Nagasawa, H ;

Toyoda, Y ;

Shinagawa, M .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1997, 233 (03) :650-654

[6] RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISMS OF THE SCRAPIE-ASSOCIATED FIBRIL PROTEIN (PRP) GENE AND THEIR ASSOCIATION WITH SUSCEPTIBILITY TO NATURAL SCRAPIE IN BRITISH SHEEP [J].

HUNTER, N ;

FOSTER, JD ;

BENSON, G ;

HOPE, J .

JOURNAL OF GENERAL VIROLOGY, 1991, 72 :1287-1292

[7] FREQUENCIES OF PRP GENE VARIANTS IN HEALTHY CATTLE AND CATTLE WITH BSE IN SCOTLAND [J].

HUNTER, N ;

GOLDMANN, W ;

SMITH, G ;

HOPE, J .

VETERINARY RECORD, 1994, 135 (17) :400-403

[8] LINKAGE OF THE GENE FOR THE SCRAPIE-ASSOCIATED FIBRIL PROTEIN (PRP) TO THE SIP GENE IN CHEVIOT SHEEP [J].

HUNTER, N ;

FOSTER, JD ;

DICKINSON, AG ;

HOPE, J .

VETERINARY RECORD, 1989, 124 (14) :364-366

[9] Characterization of the bovine prion protein gene: The expression requires interaction between the promoter and intron [J].

Inoue, S ;

Tanaka, M ;

Horiuchi, M ;

Ishiguro, N ;

Shinagawa, M .

JOURNAL OF VETERINARY MEDICAL SCIENCE, 1997, 59 (03) :175-183

[10]

Kimberlin R H, 1992, Rev Sci Tech, V11, P347