Auxin-binding-protein antibodies and peptides influence stomatal opening and alter cytoplasmic pH

被引:32
作者
Gehring, CA
McConchie, RM
Venis, MA
Parish, RW [1 ]
机构
[1] La Trobe Univ, Dept Bot, Bundoora, Vic 3083, Australia
[2] Hort Res Int, Wellesbourne CV35 9EF, Warwick, England
基金
澳大利亚研究理事会; 英国生物技术与生命科学研究理事会;
关键词
alkalinization; auxin receptor; cytoplasmic pH; guard cell; Paphiopedilum; stomatal opening;
D O I
10.1007/s004250050359
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Previous work has shown that stomatal opening induced by indole-3-acetic acid (IAA) in epidermal strips of the orchid Paphiopedilum tonsum L. is preceded by a reduction in cytoplasmic pH (pH(i)) of the guard cells. We now report that Fab fragments of an auxin-agonist antibody (D16), directed against a putative auxin-binding domain of the auxin-binding protein ABP1, induce stomatal opening and decrease guard-cell pHi, as monitored with the acetomethoxy eater of the ratiometric pH indicator Snarf-l. Similar activity was shown by a monoclonal antibody against the same domain. The C-terminal dodecapeptide, Pz152-163 of maize ABP1 (ABPzm1) induced guard-cell alkalinization and closed stomata, as did Fab fragments of a monoclonal antibody (MAC 256) recognising the C-terminal region of ABPzml. By implicating, for the first time, an auxin-binding protein in mediation of an auxin-dependent physiological response, these findings strongly support an auxin-receptor role for ABP1.
引用
收藏
页码:581 / 586
页数:6
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