The complete coding sequence of a European isolate of GB-C hepatitis G virus

被引:4
作者
Charrel, RN [1 ]
Attoui, H [1 ]
De Micco, P [1 ]
de Lamballerie, X [1 ]
机构
[1] Fac Med Marseille, Lab Virol Mol Transfusionnelle & Trop, F-13385 Marseille, France
关键词
GB virus C; hepatitis G virus; polyprotein;
D O I
10.1006/bbrc.1999.0205
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We determined the full-length coding sequence of a GB-C/hepatitis G (GBV-C/HGV) virus isolate (AUQ) obtained from a French blood donor. The genome of AUQ strain contains a long open reading frame encoding 2842 amino acid residues. Comparison with the 33 complete genome sequences so far available from the databases indicates that the full-length sequence of GBV-C/HGV AUQ showed 9.0-14.1% nucleotide sequence divergence but only 1.6-5.3% at the amino acid level. Analysis of the potential cleavage sites of the polyprotein found that they were remarkably conserved among all sequences. Although phylogenetic studies based on partial genomic sequences suggested a clusterization according to the geographical origin, analysis based on full-length polyprotein did not provide similar conclusions. (C) 1999 Academic Press.
引用
收藏
页码:432 / 437
页数:6
相关论文
共 31 条
[1]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[2]   DETECTION OF A TRYPSIN-LIKE SERINE PROTEASE DOMAIN IN FLAVIVIRUSES AND PESTIVIRUSES [J].
BAZAN, JF ;
FLETTERICK, RJ .
VIROLOGY, 1989, 171 (02) :637-639
[3]   Hepatitis G virus encodes protease activities which can effect processing of the virus putative nonstructural proteins [J].
Belyaev, AS ;
Chong, S ;
Novikov, A ;
Kongpachith, A ;
Masiarz, FR ;
Lim, M ;
Kim, JP .
JOURNAL OF VIROLOGY, 1998, 72 (01) :868-872
[4]  
CANTALOUBE JF, 1998, IN PRESS TRANSFUSION
[5]   The complete coding sequence of hepatitis C virus genotype 5a, the predominant genotype in South Africa [J].
Chamberlain, RW ;
Adams, NJ ;
Taylor, LA ;
Simmonds, P ;
Elliott, RM .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1997, 236 (01) :44-49
[6]   Classification of hepatitis C virus variants in six major types based on analysis of the envelope 1 and nonstructural 5B genome regions and complete polyprotein sequences [J].
deLamballerie, X ;
Charrel, RN ;
Attoui, H ;
DeMicco, P .
JOURNAL OF GENERAL VIROLOGY, 1997, 78 :45-51
[7]   Molecular cloning and characterization of a GB virus C isolate from a patient with non-A-E hepatitis [J].
Erker, JC ;
Simons, JN ;
Muerhoff, AS ;
Leary, TP ;
Chalmers, ML ;
Desai, SM ;
Mushahwar, IK .
JOURNAL OF GENERAL VIROLOGY, 1996, 77 :2713-2720
[8]   Nucleotide sequence of the 5' noncoding region of hepatitis G virus isolated from Japanese patients: Comparison with reported isolates [J].
Fukushi, S ;
Kurihara, C ;
Ishiyama, N ;
Okamura, H ;
Hoshino, FB ;
Oya, A ;
Katayama, K .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1996, 226 (02) :314-318
[9]  
KAMER G, 1984, NUCLEIC ACIDS RES, V12, P7269, DOI 10.1093/nar/12.18.7269
[10]   Full-length GBV-C/HGV genomes from nine Japanese isolates: characterization by comparative analyses [J].
Katayama, K ;
Kageyama, T ;
Fukushi, S ;
Hoshino, FB ;
Kurihara, C ;
Ishiyama, N ;
Okamura, H ;
Oya, A .
ARCHIVES OF VIROLOGY, 1998, 143 (06) :1063-1075