Phenotypic, Biochemical, and Molecular Techniques for Detection of Metallo-β-Lactamase NDM in Acinetobacter baumannii

被引：57

作者：

Bonnin, Remy A.

Naas, Thierry

Poirel, Laurent

Nordmann, Patrice ^{[1
]}

机构：

[1] Hop Bicetre, AP HP, Fac Med, INSERM U914,Serv Bacteriol Virol, K Bicetre, France

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2012年 / 50卷 / 04期

关键词：

RESISTANCE; CARBAPENEMASE; SPREAD; SPP;

D O I：

10.1128/JCM.06276-11

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Seven NDM-positive Acinetobacter baumannii isolates of worldwide origin were studied to evaluate the best technique for their identification. Detection of carbapenemase producers based on the measurement of carbapenemase activity by UV spectrophotometry (as for A. baumannii strains producing other types of carbapenemase), or by the modified Hodge test, failed. Inhibition activity using EDTA was a sensitive technique but lacked specificity compared to molecular techniques, which remain the gold standard.

引用

页码：1419 / 1421

页数：3

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