Agrobacterium rhizogenes-mediated transformation of Sesbania rostrata

被引：17

作者：

Van de Velde, W ^{[1
]}

Mergeay, J ^{[1
]}

Holsters, M ^{[1
]}

Goormachtig, S ^{[1
]}

机构：

[1] Univ Ghent, Dept Plant Syst Biol, B-9052 Ghent, Belgium

来源：

PLANT SCIENCE | 2003年 / 165卷 / 06期

关键词：

Agrobacterium rhizogenes; nodulation; Sesbania rostrata; transformation;

D O I：

10.1016/S0168-9452(03)00339-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In order to study the function of nodulin genes and the activity of their promoters, the Agrobacterium rhizogenes transformation strategy was adopted for Sesbania rostrata. Two protocols were selected to generate S. rostrata transgenic roots that can be nodulated efficiently after application of Azorhizobium caulinodans, ORS571. Cotransformation frequencies of 22 and 72% were obtained with the first and second protocol, respectively. The transgenic root nodules showed no apparent differences when compared with the wild-type root nodules. The screening procedure for cotransformation of binary T-DNA in transgenic roots and root nodules was optimized by using an enhanced green-fluorescent protein construct. The A. rhizogenes transformation system was used to analyze the 35S promoter-driven expression of reporter genes in developing root nodules. (C) 2003 Elsevier Ireland Ltd. All rights reserved.

引用

页码：1281 / 1288

页数：8

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