Switch-based mechanism of kinesin motors

被引:293
作者
Kikkawa, M
Sablin, EP
Okada, Y
Yajima, H
Fletterick, RJ
Hirokawa, N
机构
[1] Univ Tokyo, Grad Sch Med, Dept Cell Biol & Anat, Bunkyo Ku, Tokyo 1130033, Japan
[2] Univ Calif San Francisco, Dept Biochem Biophys, San Francisco, CA 94143 USA
关键词
D O I
10.1038/35078000
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Kinesin motors are specialized enzymes that use hydrolysis of ATP to generate force and movement along their cellular tracks, the microtubules. Although numerous biochemical and biophysical studies have accumulated much data that link microtubule-assisted ATP hydrolysis to kinesin motion, the structural view of kinesin movement remains unclear. This study of the monomeric kinesin motor KIF1A combines X-ray crystallography and cryo-electron microscopy, and allows analysis of force-generating conformational changes at atomic resolution. The motor is revealed in its two functionally critical states-complexed with ADP and with a non-hydrolysable analogue of ATP. The conformational change observed between the ADP-bound and the ATP-like structures of the KIF1A catalytic core is modular, extends to all kinesins and is similar to the conformational change used by myosin motors and G proteins. Docking of the ADP-bound and ATP-like crystallographic models of KIF1A into the corresponding cryo-electron microscopy maps suggests a rationale for the plus-end directional bias associated with the kinesin catalytic core.
引用
收藏
页码:439 / 445
页数:7
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