Role of phosphorylation clusters in the biology of the coronavirus infectious bronchitis virus nucleocapsid protein

被引：38

作者：

Spencer, Kelly-Anne ^{[1
]}

Dee, Michael ^{[2
]}

Britton, Paul ^{[2
]}

Hiscox, Julian A. ^{[1
,3
]}

机构：

[1] Univ Leeds, Fac Biol Sci, Inst Mol & Cellular Biol, Leeds LS2 9JT, W Yorkshire, England

[2] Inst Anim Hlth, Div Microbiol, Newbury RG20 7NN, Berks, England

[3] Univ Leeds, Astbury Ctr Struct Mol Biol, Leeds LS2 9JT, W Yorkshire, England

来源：

VIROLOGY | 2008年 / 370卷 / 02期

基金：

英国生物技术与生命科学研究理事会;

关键词：

coronavirus; RNA binding; nucleocapsid protein; IBV; surface plasmon resonance; phosphorylation;

D O I：

10.1016/j.virol.2007.08.016

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The coronavirus infectious bronchitis virus (IBV) nucleocapsid (N) protein is an RNA binding protein which is phosphorylated at two conserved clusters. Kinetic analysis of RNA binding indicated that the C-terminal phosphorylation cluster was involved in the recognition of viral RNA from non-viral RNA. The IBV N protein has been found to be essential for the successful recovery of IBV using reverse genetics systems. Rescue experiments indicated that phosphorylated N protein recovered infectious IBV more efficiently when compared to modified N proteins either partially or non-phosphorylated. Our data indicate that the phosphorylated form of the IBV N protein plays a role in virus biology. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：373 / 381

页数：9

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