miR-206 and -486 Induce Myoblast Differentiation by Downregulating Pax7

被引：339

作者：

Dey, Bijan K. ^{[1
]}

Gagan, Jeffrey ^{[1
]}

Dutta, Anindya ^{[1
]}

机构：

[1] Univ Virginia, Sch Med, Dept Biochem & Mol Genet, Charlottesville, VA 22908 USA

来源：

MOLECULAR AND CELLULAR BIOLOGY | 2011年 / 31卷 / 01期

关键词：

STEM-CELL BEHAVIOR; SATELLITE CELLS; MUSCLE; EXPRESSION; MICRORNAS; GENES; MYOGENESIS; MECHANISM; MYOD; PROLIFERATION;

D O I：

10.1128/MCB.01009-10

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Pax7 transcription factor is required for muscle satellite cell biogenesis and specification of the myogenic precursor lineage. Pax7 is expressed in proliferating myoblasts but is rapidly downregulated during differentiation. Here we report that miR-206 and -486 are induced during myoblast differentiation and downregulate Pax7 by directly targeting its 3' untranslated region (UTR). Expression of either of these microRNAs in myoblasts accelerates differentiation, whereas inhibition of these microRNAs causes persistence of Pax7 protein and delays differentiation. A microRNA-resistant form of Pax7 is sufficient to inhibit differentiation. Since both these microRNAs are induced by MyoD and since Pax7 promotes the expression of Id2, an inhibitor of MyoD, our results revealed a bistable switch that exists either in a Pax7-driven myoblast state or a MyoD-driven myotube state.

引用

页码：203 / 214

页数：12

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