Detection of NADH and ethanol at titanium containing MCM-41 with low overpotential

被引：15

作者：

Dai, Zhihui ^{[1
]}

Lu, Guofei

Bao, Jianchun

Huang, Xiaohua

Ju, Huangxian

机构：

[1] Nanjing Normal Univ, Dept Chem, Nanjing 210097, Peoples R China

[2] Nanjing Univ, Dept Chem, Nanjing 210093, Peoples R China

[3] Nanjing Xiaozhuang Coll, Dept Chem, Nanjing 210017, Peoples R China

来源：

ELECTROANALYSIS | 2007年 / 19卷 / 15期

关键词：

NADH; Ti-MCM-41; electrocatalytic; sensors; ADH; ethanol;

D O I：

10.1002/elan.200703888

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Titanium-containing MCM-41 (Ti-MCM-41) modified glassy carbon electrode (GCE) can exhibit an excellent electrocatalytic activity towards the oxidation of P-Nicotinamide adenine dinucleotide (NADH). A dramatic decrease in the overvoltage of NADH oxidation reaction is observed at 0.28 V vs. SCE. The application in the amperometric biosensing of ethanol using alcohol dehydrogenase enzyme (ADH) also has been demonstrated with this material. The proposed sensor shows a highly sensitivity, an acceptable reproducibility and a good stability. The linear range of ethanol is 25 - 1.000 mu M and the detection limit is 8.0 mu M. Ti-MCM-41 modified electrode not only can be used to detect the concentration of NADH in biochemical reaction, but also as the potential matrix for the construction of dehydrogenases sensor.

引用

页码：1591 / 1596

页数：6

共 35 条

[1] Electrocatalytic detection of NADH and glycerol by NAD+-modified carbon electrodes [J].

Alvarez-González, MI ;

Saidman, SA ;

Lobo-Castañón, MJ ;

Miranda-Ordieres, AJ ;

Tuñón-Blanco, P .

ANALYTICAL CHEMISTRY, 2000, 72 (03) :520-527

[2]

BEATRIU PS, 2004, BIOSENS BIOELECTRON, V19, P1131

[3] STUDY OF ELECTROCHEMICAL OXIDATION OF REDUCED NICOTINAMIDE ADENINE-DINUCLEOTIDE [J].

BLAEDEL, WJ ;

JENKINS, RA .

ANALYTICAL CHEMISTRY, 1975, 47 (08) :1337-1343

[4] Assembling and evaluation of new dehydrogenase enzyme electrode probes obtained by electropolymerization of aminobenzene isomers and PQQ on gold, platinum and carbon electrodes [J].

Curulli, A ;

Carelli, I ;

Trischitta, O ;

Palleschi, G .

BIOSENSORS & BIOELECTRONICS, 1997, 12 (9-10) :1043-1055

[5] Direct electron transfer and enzymatic activity of hemoglobin in a hexagonal mesoporous silica matrix [J].

Dai, ZH ;

Liu, SQ ;

Ju, HX ;

Chen, HY .

BIOSENSORS & BIOELECTRONICS, 2004, 19 (08) :861-867

[6] Low potential detection of NADH at titanium-containing MCM-41 modified glassy carbon electrode [J].

Dai, Zhihui ;

Lu, Guofei ;

Bao, Jianchun ;

Huang, Xiaohua ;

Ju, Huangxian .

ELECTROANALYSIS, 2007, 19 (05) :604-607

[7] Reactivity of poly(anilineboronic acid) with NAD+ and NADH [J].

Deore, LA ;

Freund, MS .

CHEMISTRY OF MATERIALS, 2005, 17 (11) :2918-2923

[8]

Gorton Lo, 2002, J Biotechnol, V82, P371, DOI 10.1016/S1389-0352(01)00053-8

[9] Sol-gel processed TiO2 films for photovoltaic applications [J].

Grätzel, M .

JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY, 2001, 22 (1-2) :7-13

[10] Preservation of NADH voltammetry for enzyme-modified electrodes based on dehydrogenase [J].

Hayes, MA ;

Kuhr, WG .

ANALYTICAL CHEMISTRY, 1999, 71 (09) :1720-1727

← 1 2 3 4 →