Micropropagation of the endangered Aloe polyphylla

被引：65

作者：

Abrie, AL ^{[1
]}

van Staden, J ^{[1
]}

机构：

[1] Univ Natl Pietermaritzburg, Sch Bot & Zool, Res Ctr Plant Growth & Dev, ZA-3209 Scottsville, South Africa

来源：

PLANT GROWTH REGULATION | 2001年 / 33卷 / 01期

基金：

新加坡国家研究基金会;

关键词：

aloe polyphylla; benzyladenine; conservation; micropropagation;

D O I：

10.1023/A:1010725901900

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A rapid propagation protocol was establish for the highly endangered Aloe polyphylla (Schonland ex Pillans). Seed was germinated in vitro on Murashige & Skoog 1962 medium [6] with or without sucrose. Plantlets were cultured on medium containing benzyladenine only, or a combination of BA and NAA. After initial problems with browning, the explants rapidly formed axillary and adventitious buds. Maximal shoot formation was obtained on MS medium containing 1.0 mg l(-1) BA. Some shoots rooted spontaneously on MS medium, but the rooting percentage was improved with a 0.5 mg l(-1) IBA supplement. Rooted plantlets were acclimatized to greenhouse conditions. The success of this project indicates that micropropagation can be a useful tool in the conservation of this endangered species of the genus Aloe.

引用

页码：19 / 23

页数：5

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