Effect of sciatic nerve crush on local and target tissue production of neurotrophin-3 transcripts in rats

被引：17

作者：

Cai, F ^{[1
]}

Tomlinson, DR ^{[1
]}

Fernyhough, F ^{[1
]}

机构：

[1] Univ London Queen Mary & Westfield Coll, Dept Pharmacol, St Bartholomews & Royal London Hosp, Sch Med & Dent, London E1 4NS, England

来源：

NEUROSCIENCE LETTERS | 1998年 / 252卷 / 01期

基金：

英国惠康基金;

关键词：

nerve growth factors; neurotrophins; skin; axotomy; cRT-PCR; Schwann cell;

D O I：

10.1016/S0304-3940(98)00543-6

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The effect of sciatic nerve crush in adult rats on neurotrophin-3 (NT-3) mRNA expression at the site of crush and in ipsilateral foot skin was studied using competitive reverse transcription-polymerase chain reaction (cRT-PCR). Mid-sciatic nerve crush resulted in a significant reduction in the expression of NT-3 mRNA in nerve segments distal to the injury site at 3 and 7 days (approximate 60% decrease; P < 0.01). The reduced NT-3 mRNA expression started to increase at days 14 post-crush and returned towards control levels at 21 days following the crush. The nerve segment proximal to the crush site showed a similar changed pattern of NT-3 mRNA expression. The effects of denervation on NT-3 mRNA expression in foot skin were also studied. Reduced expression of NT-3 was observed by 7 days post-crush, with a 25% decrease observed by 14 days (P < 0.002). Levels of NT-3 mRNA had returned to normal by 21 days post-crush, The results show that changes in axon-Schwann cell contact do not account for the nerve crush induced loss, or subsequent recovery, of NT-3 mRNA expression in nerve. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：45 / 48

页数：4

共 27 条

[1] Specific subtypes of cutaneous mechanoreceptors require neurotrophin-3 following peripheral target innervation [J].