Inhibition of phosphofructokinases by copper(II)

被引：10

作者：

Gebhard, S ^{[1
]}

Ronimus, RS ^{[1
]}

Morgan, HW ^{[1
]}

机构：

[1] Univ Waikato, Dept Biol Sci, Thermophile Res Unit, Hamilton, New Zealand

来源：

FEMS MICROBIOLOGY LETTERS | 2001年 / 197卷 / 01期

关键词：

phosphofructokinase; copper(II); toxicity; thermophilic; glycolysis;

D O I：

10.1016/S0378-1097(01)00094-5

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The biochemical inhibition by Cu2+ on eight phylogenetically and biochemically different phosphofructokinases (PFKs) was investigated. The enzymes screened included representatives from thermophilic and mesophilic bacteria, a hyperthermophilic archaeon and a eukaryote. covering all three phosphoryl donor subtypes (ATP, ADP and pyrophosphate). The sensitivities of the enzymes to Cut varied greatly. with the archaeal ADP-PFK being the least and the eukaryote ATP-PFK being the most sensitive. The bacterial ATP- and pyrophosphate-dependent PFKs showed intermediate sensitivity with the exception of the Spirochaeta thermophila enzyme (pyrophosphate-dependent) which was relatively resistant. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：105 / 109

页数：5

共 16 条

[1] KINETIC CHARACTERISTICS OF PHOSPHOFRUCTOKINASE FROM BACILLUS-STEAROTHERMOPHILUS - MGATP NONALLOSTERICALLY INHIBITS THE ENZYME [J].