Molecular phylogeny of the genus Pseudomonas based on rpoB sequences and application for the identification of isolates

被引：205

作者：

Tayeb, LA ^{[1
]}

Ageron, E ^{[1
]}

Grimont, F ^{[1
]}

Grimont, PAD ^{[1
]}

机构：

[1] Inst Pasteur, INSERM, Unite Biodiversite Bacteries Pathogenes Emergente, Unit 389, F-75724 Paris, France

来源：

RESEARCH IN MICROBIOLOGY | 2005年 / 156卷 / 5-6期

关键词：

Pseudomonas; phylogeny; rpoB; sequencing;

D O I：

10.1016/j.resmic.2005.02.009

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Phylogenetic relationships within the genus Pseudomonas were examined by comparing partial (about 1000 nucleotides) rpoB gene sequences. A total of 186 strains belonging to 75 species of Pseudomonas sensu stricto and related species were studied. The phylogenetic resolution of the rpoB tree was approximately three times higher than that of the rrs tree. Ribogroups published earlier correlated well with rpoB sequence clusters. The rpoB sequence database generated by this study was used for identification. A total of 89 isolates (79.5%) were identified to a named species, while 16 isolates (14.3%) corresponded to unnamed species, and 7 isolates (6.2%) had uncertain affiliation. rpoB sequencing is now being used for routine identification of Pseudomonas isolates in our laboratory. (c) 2005 Elsevier SAS. All rights reserved.

引用

页码：763 / 773

页数：11

共 29 条

[1] The phylogeny of the genera Chryseomonas, Flavimonas, and Pseudomonas supports synonymy of these three genera [J].

Anzai, Y ;

Kudo, Y ;

Oyaizu, H .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1997, 47 (02) :249-251

[2] Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence [J].

Anzai, Y ;

Kim, H ;

Park, JY ;

Wakabayashi, H ;

Oyaizu, H .

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2000, 50 :1563-1589

[3]

BORODIN AM, 1988, DOKL AKAD NAUK SSSR+, V302, P1261

[4] Taxonomic diversity of pseudomonads revealed by computer-interpretation of ribotyping data [J].

Brosch, R ;

Lefevre, M ;

Grimont, F ;

Grimont, PAD .

SYSTEMATIC AND APPLIED MICROBIOLOGY, 1996, 19 (04) :541-555

[5] rpoB-based microbial community analysis avoids limitations inherent in 16S rRNA gene intraspecies heterogeneity [J].

Dahllöf, I ;

Baillie, H ;

Kjelleberg, S .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (08) :3376-3380

[6] Sequence diversity of the oprI gene, coding for major outer membrane lipoprotein I, among rRNA group I pseudomonads [J].

De Vos, D ;

Bouton, C ;

Sarniguet, A ;

De Vos, P ;

Vauterin, M ;

Cornelis, P .

JOURNAL OF BACTERIOLOGY, 1998, 180 (24) :6551-6556

[7] INTRAGENERIC AND INTERGENERIC SIMILARITIES OF PSEUDOMONAS AND XANTHOMONAS RIBOSOMAL RIBONUCLEIC-ACID CISTRONS [J].

DEVOS, P ;

DELEY, J .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1983, 33 (03) :487-509

[8] Detection of 400-year-old Yersinia pestis DNA in human dental pulp:: An approach to the diagnosis of ancient septicemia [J].

Drancourt, M ;

Aboudharam, G ;

Signoli, M ;

Dutour, O ;

Raoult, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (21) :12637-12640

[9] HOW CLOSE IS CLOSE - 16S RIBOSOMAL-RNA SEQUENCE IDENTITY MAY NOT BE SUFFICIENT TO GUARANTEE SPECIES IDENTITY [J].

FOX, GE ;

WISOTZKEY, JD ;

JURTSHUK, P .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1992, 42 (01) :166-170

[10] Ability of Biolog and Biotype-100 systems to reveal the taxonomic diversity of the pseudomonads [J].

Grimont, PAD ;

Vancanneyt, M ;

Lefevre, M ;

Vandemeulebroecke, K ;

Vauterin, L ;

Brosch, R ;

Kersters, K ;

Grimont, F .

SYSTEMATIC AND APPLIED MICROBIOLOGY, 1996, 19 (04) :510-527

← 1 2 3 →