Sequence diversity of the oprI gene, coding for major outer membrane lipoprotein I, among rRNA group I pseudomonads

被引:25
作者
De Vos, D
Bouton, C
Sarniguet, A
De Vos, P
Vauterin, M
Cornelis, P
机构
[1] Free Univ Brussels VIB, Dept Immunol Parasitol & Ultrastruct, B-1640 Rhode St Genese, Belgium
[2] Free Univ Brussels VIB, Lab Plant Genet, B-1640 Rhode St Genese, Belgium
[3] Univ Ghent, Microbiol Lab, B-9000 Ghent, Belgium
[4] INRA, Ctr Rech Rennes, Pathol Vegetale Stn, F-35650 Le Rheu, France
关键词
D O I
10.1128/JB.180.24.6551-6556.1998
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The sequence of oprI, the gene coding for the major outer membrane lipoprotein I, was determined by PCR sequencing for representatives of 17 species of rRNA group I pseudomonads, with a special emphasis on Pseudomonas aeruginosa and Pseudomonas fluorescens. Within the P. aeruginosa species, oprI sequences for 25 independent isolates were found to be identical, except for one silent substitution at position 96. The oprI sequences diverged more for the other rRNA group I pseudomonads (85 to 91% similarity,vith P. aeruginosa oprI). An accumulation of silent and also (but to a much lesser extent) nonsilent substitutions in the different sequences was found. A clustering according to the respective presence and/or positions of the Haem, PvuII, and SghI sites could also be obtained. A sequence cluster analysis showed a rather widespread distribution of P. fluorescens isolates. All other rRNA group I pseudomonads clustered in a manner that was in agreement with other studies, showing that the oprI gene can be useful as a complementary phylogenetic marker for classification of rRNA group I pseudomonads.
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页码:6551 / 6556
页数:6
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