Pre-unfolding resonant oscillations of single green fluorescent protein molecules

被引：48

作者：

Baldini, G ^{[2
]}

Cannone, F

Chirico, G

机构：

[1] Univ Milano Bicocca, INFM, CNR, I-20126 Milan, Italy

[2] Univ Milano Bicocca, Dept Phys, Lab Adv Biospectroscopy, I-20126 Milan, Italy

来源：

SCIENCE | 2005年 / 309卷 / 5737期

关键词：

D O I：

10.1126/science.1115001

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Fluorescence spectroscopy of a green fluorescent protein mutant at single-molecule resolution has revealed a remarkable oscillatory behavior that can also be driven by applied fields. We show that immediately before unfolding, several periodic oscillations among the chemical substates of the protein chromophore occur. We also show that applied alternating electric or acoustic fields, when tuned to the protein characteristic frequencies, give rise to strong resonance effects.

引用

页码：1096 / 1100

页数：5

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