Biological phosphate ester sensing using an artificial enzyme PMP complex

被引：10

作者：

Ikeno, S

Haruyama, T

机构：

[1] Kyushu Inst Technol, Dept Biol Funct & Engn, Kitakyushu, Fukuoka 8080196, Japan

[2] Japan Sci & Technol Agcy, CREST, Kawaguchi, Saitama 3320012, Japan

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2005年 / 108卷 / 1-2期

关键词：

sensor; artificial enzyme; biological phosphate ester; dephosphorylation; PO43-; electrochemical detection; polyion complex;

D O I：

10.1016/j.snb.2004.11.077

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Biological phosphate ester (phosphoric ester) substances, e.g. ATP, ADP, AMP, pyrophosphate and deoxyribonucleic acid, play important roles as either energy-carrying substances or substances carrying biological information. The author has designed and synthesized an artificial enzyme PMP complex (Cu) which dephosphorylates phosphate ester substances. The synthesized PMP complex (Cu) showed a prominent dephosphorylating effect on biological phosphate ester substances, e.g. ATP, ADP, AMP and pyrophosphate. A PMP complex (Cu)-coated electrode was employed as a sensor device. A current response could be obtained as a reaction to the application of phosphate ester substances on the sensor when a constant potential of -250 mV versus Ag/AgCl was applied. In the case of ATP, the sensor could determine the ATP concentration within the range between 1 nM and 20 mM, and could also determine the presence of other biological phosphate ester substances. (c) 2004 Elsevier B.V. All rights reserved.

引用

页码：608 / 612

页数：5

共 15 条

[1] Metallocyclodextrin catalysts for hydrolysis of phosphate triesters [J].