Sorbitol-mediated stabilization of human IgG against thermal inactivation

被引：9

作者：

Cioci, F ^{[1
]}

Lavecchia, R ^{[1
]}

机构：

[1] Univ Roma La Sapienza, Dept Chem Engn, I-00184 Rome, Italy

来源：

BIOTECHNOLOGY TECHNIQUES | 1998年 / 12卷 / 11期

关键词：

immunoglobulins; sorbitol; protein denaturation; stabilization;

D O I：

10.1023/A:1008839912640

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Sorbitol at 30% (w/w) stabilized human IgG to thermal denaturation from 60 to 85 degrees C, increasing the protein's half life from 25 to 266 min at 70 degrees C. A kinetic model based on the Lumry-Eyring inactivation scheme was developed and used to estimate the apparent rate constant and activation energy.

引用

页码：855 / 858

页数：4

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