Fusobacterium nucleatum infection is prevalent in human colorectal carcinoma

被引：1474

作者：

Castellarin, Mauro ^{[1
,2
]}

Warren, Rene L. ^{[1
]}

Freeman, J. Douglas ^{[1
]}

Dreolini, Lisa ^{[1
]}

Krzywinski, Martin ^{[1
]}

Strauss, Jaclyn ^{[3
]}

Barnes, Rebecca ^{[4
]}

Watson, Peter ^{[4
]}

Allen-Vercoe, Emma ^{[3
]}

Moore, Richard A. ^{[1
,5
]}

Holt, Robert A. ^{[1
,2
]}

机构：

[1] Michael Smith Genome Sci Ctr, BC Canc Agcy, Vancouver, BC V5Z 1L3, Canada

[2] Simon Fraser Univ, Dept Mol Biol & Biochem, Burnaby, BC V5A 1S6, Canada

[3] Univ Guelph, Guelph, ON N1G 2W1, Canada

[4] Deeley Res Ctr, BC Canc Agcy, Victoria, BC V8R 6V5, Canada

[5] Simon Fraser Univ, Fac Hlth Sci, Burnaby, BC V5A 1S6, Canada

来源：

GENOME RESEARCH | 2012年 / 22卷 / 02期

基金：

加拿大健康研究院;

关键词：

ORAL EPITHELIAL-CELLS; BRAIN-ABSCESS; BACTERIA; HEALTH; SEQUENCES; PRIMERS; CATALOG;

D O I：

10.1101/gr.126516.111

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An estimated 15% or more of the cancer burden worldwide is attributable to known infectious agents. We screened colorectal carcinoma and matched normal tissue specimens using RNA-seq followed by host sequence subtraction and found marked over-representation of Fusobacterium nucleatum sequences in tumors relative to control specimens. F. nucleatum is an invasive anaerobe that has been linked previously to periodontitis and appendicitis, but not to cancer. Fusobacteria are rare constituents of the fecal microbiota, but have been cultured previously from biopsies of inflamed gut mucosa. We obtained a Fusobacterium isolate from a frozen tumor specimen; this showed highest sequence similarity to a known gut mucosa isolate and was confirmed to be invasive. We verified overabundance of Fusobacterium sequences in tumor versus matched normal control tissue by quantitative PCR analysis from a total of 99 subjects (p = 2.5 x 10(-6)), and we observed a positive association with lymph node metastasis.

引用

页码：299 / 306

页数：8

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