Meiosis I is established through division-specific translational control of a cyclin

被引：161

作者：

Carlile, Thomas M. ^{[1
,2
]}

Amon, Angelika ^{[1
,2
]}

机构：

[1] MIT, Ctr Canc Res, Cambridge, MA 02139 USA

[2] MIT, Howard Hughes Med Inst, Cambridge, MA 02139 USA

来源：

CELL | 2008年 / 133卷 / 02期

关键词：

D O I：

10.1016/j.cell.2008.02.032

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In budding yeast, key meiotic events such as DNA replication, recombination, and the meiotic divisions are controlled by Clb cyclin-dependent kinases (Clb-CDKs). Using a novel synchronization procedure, we have characterized the activity of these Clb-CDKs and observed a surprising diversity in their regulation during the meiotic divisions. Clb1-CDK activity is restricted to meiosis I, and Clb3-CDK activity to meiosis II, through 5'UTR-mediated translational control of its transcript. The analysis of cells inappropriately producing Clb3-CDKs during meiosis I furthermore defines Clb3 as an inhibitor of the meiosis I chromosome segregation program. Our results demonstrate an essential role for Clb-CDK regulation in establishing the meiotic chromosome segregation pattern.

引用

页码：280 / 291

页数：12

共 40 条

[1] Control of landmark events in meiosis by the CDK Cdc28 and the meiosis-specific kinase Ime2 [J].

Benjamin, KR ;

Zhang, C ;

Shokat, KM ;

Herskowitz, I .

GENES & DEVELOPMENT, 2003, 17 (12) :1524-1539

[2] Multiple levels of cyclin specificity in cell-cycle control [J].

Bloom, Joanna ;

Cross, Frederick R. .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2007, 8 (02) :149-160

[3] Division of the nucleolus and its release of CDC14 during anaphase of meiosis I depends on separase, SPO12, and SLK19 [J].

Buonomo, SBC ;

Rabitsch, KP ;

Fuchs, J ;

Gruber, S ;

Sullivan, M ;

Uhlmann, F ;

Petronczki, M ;

Tóth, A ;

Nasmyth, K .

DEVELOPMENTAL CELL, 2003, 4 (05) :727-739

[4] The transcriptional program of sporulation in budding yeast [J].

Chu, S ;

DeRisi, J ;

Eisen, M ;

Mulholland, J ;

Botstein, D ;

Brown, PO ;

Herskowitz, I .

SCIENCE, 1998, 282 (5389) :699-705

[5] Anaphase initiation in Saccharomyces cerevisiae is controlled by the APC-dependent degradation of the anaphase inhibitor Pds1p [J].

CohenFix, O ;

Peters, JM ;

Kirschner, MW ;

Koshland, D .

GENES & DEVELOPMENT, 1996, 10 (24) :3081-3093

[6]

COLLART MA, 1993, CURRENT PROTOCOLS MO, V2

[7] General translational repression by activators of mRNA decapping [J].

Coller, J ;

Parker, R .

CELL, 2005, 122 (06) :875-886

[8] A POTENTIAL POSITIVE FEEDBACK LOOP CONTROLLING CLN1 AND CLN2 GENE-EXPRESSION AT THE START OF THE YEAST-CELL CYCLE [J].

CROSS, FR ;

TINKELENBERG, AH .

CELL, 1991, 65 (05) :875-883

[9]

DAHMANN C, 1995, GENETICS, V140, P957

[10] A high-resolution map of transcription in the yeast genome [J].

David, L ;

Huber, W ;

Granovskaia, M ;

Toedling, J ;

Palm, CJ ;

Bofkin, L ;

Jones, T ;

Davis, RW ;

Steinmetz, LM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (14) :5320-5325

← 1 2 3 4 →