Messenger RNA regulation: to translate or to degrade

被引:364
作者
Shyu, Ann-Bin [1 ]
Wilkinson, Miles F. [2 ]
van Hoof, Ambro [3 ]
机构
[1] Univ Texas Houston, Sch Med, Dept Biochem & Mol Biol, Houston, TX 77030 USA
[2] Univ Texas Houston, MD Anderson Canc Ctr, Dept Biochem & Mol Biol, Houston, TX 77030 USA
[3] Univ Texas Houston, Sch Med, Dept Microbiol & Mol Genet, Houston, TX USA
关键词
microRNA; mRNA decay; NMD; P-bodies; translation;
D O I
10.1038/sj.emboj.7601977
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Quality control of gene expression operates post-transcriptionally at various levels in eukaryotes. Once transcribed, mRNAs associate with a host of proteins throughout their lifetime. These mRNA-protein complexes (mRNPs) undergo a series of remodeling events that are influenced by and/or influence the translation and mRNA decay machinery. In this review we discuss how a decision to translate or to degrade a cytoplasmic mRNA is reached. Nonsense-mediated mRNA decay (NMD) and microRNA (miRNA)-mediated mRNA silencing are provided as examples. NMD is a surveillance mechanism that detects and eliminates aberrant mRNAs whose expression would result in truncated proteins that are often deleterious to the organism. miRNA-mediated mRNA silencing is a mechanism that ensures a given protein is expressed at a proper level to permit normal cellular function. While NMD and miRNA-mediated mRNA silencing use different decision-making processes to determine the fate of their targets, both are greatly influenced by mRNP dynamics. In addition, both are linked to RNA processing bodies. Possible modes involving 30 untranslated region and its associated factors, which appear to play key roles in both processes, are discussed.
引用
收藏
页码:471 / 481
页数:11
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