How to study proteins by circular dichroism

被引:2620
作者
Kelly, SM [1 ]
Jess, TJ [1 ]
Price, NC [1 ]
机构
[1] Univ Glasgow, Inst Biomed & Life Sci, Div Biochem & Mol Biol, Glasgow G12 8QQ, Lanark, Scotland
来源
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS | 2005年 / 1751卷 / 02期
基金
英国生物技术与生命科学研究理事会;
关键词
circular dichroism; protein structure; secondary structure; protein folding; ligand binding;
D O I
10.1016/j.bbapap.2005.06.005
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Circular dichroism (CD) is being increasingly recognised as a valuable technique for examining the structure of proteins in solution. However, the value of many studies using CD is compromised either by inappropriate experimental design or by lack of attention to key aspects of instrument calibration or sample characterisation. In this article, we summarise the basis of the CD approach and its application to the study of proteins, and then present clear guidelines on how reliable data can be obtained and analysed. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:119 / 139
页数:21
相关论文
共 85 条
[51]   The structural basis of ribosome activity in peptide bond synthesis [J].
Nissen, P ;
Hansen, J ;
Ban, N ;
Moore, PB ;
Steitz, TA .
SCIENCE, 2000, 289 (5481) :920-930
[52]   The optimization of protein secondary structure determination with infrared and circular dichroism spectra [J].
Oberg, KA ;
Ruysschaert, JM ;
Goormaghtigh, E .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 2004, 271 (14) :2937-2948
[53]  
Pace C N, 1986, Methods Enzymol, V131, P266
[54]   HOW TO MEASURE AND PREDICT THE MOLAR ABSORPTION-COEFFICIENT OF A PROTEIN [J].
PACE, CN ;
VAJDOS, F ;
FEE, L ;
GRIMSLEY, G ;
GRAY, T .
PROTEIN SCIENCE, 1995, 4 (11) :2411-2423
[55]   Sequence and structural duality: Designing peptides to adopt two stable conformations [J].
Pandya, MJ ;
Cerasoli, E ;
Joseph, A ;
Stoneman, RG ;
Waite, E ;
Woolfson, DN .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (51) :17016-17024
[56]  
POLYANICHKO A, IN PRESS BIOPOLYMERS
[57]  
PRICE NC, 1996, DETERMINATION PROTEI, P34
[58]  
Price NC, 1999, FUNDAMENTALS ENZYMOL, P15
[59]   The key role of protein flexibility in modulating IgE interactions [J].
Price, NE ;
Price, NC ;
Kelly, SM ;
McDonnell, JM .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (03) :2324-2330
[60]   ESTIMATION OF GLOBULAR PROTEIN SECONDARY STRUCTURE FROM CIRCULAR-DICHROISM [J].
PROVENCHER, SW ;
GLOCKNER, J .
BIOCHEMISTRY, 1981, 20 (01) :33-37