Apoptosis is associated with modifications of bcl-2 mRNA AU-Binding proteins

被引：17

作者：

Donnini, M

Lapucci, A

Papucci, L

Witort, E

Tempestini, A

Brewer, G

Bevilacqua, A

Nicolin, A

Capaccioli, S ^{[1
]}

Schiavone, N

机构：

[1] Univ Florence, Sch Med, Dept Expt Pathol & Oncol, I-50121 Florence, Italy

[2] Univ Milan, Sch Med, Dept Pharmacol, I-20129 Milan, Italy

[3] Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Mol Genet & Microbiol, Piscataway, NJ 08854 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2001年 / 287卷 / 05期

关键词：

apoptosis; bcl-2; gene expression; post-transcriptional control; AU-rich elements; AU-binding proteins;

D O I：

10.1006/bbrc.2001.5700

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The expression of genes requiring finely tuned control is regulated by a posttranscriptional mechanism involving mRNA A + U-rich elements (AREs) cooperating with ARE-binding proteins (AUBPs) in modulation of mRNA stability. We reported previously that an ARE in the bcl-2 mRNA 3'-untranslated region (3'-UTR) had destabilizing activity and was involved in bcl-2 downregulation during apoptosis in vitro. Here we demonstrate that the bcl-2 ARE complexes with a number of specific AUBPs, whose pattern undergoes changes following application of apoptotic stimuli. The caspase inhibitor Z-VAD-fmk strongly attenuates both bcl-2 mRNA decay and bcl-2 AUBP pattern changes elicited by apoptotic stimuli, indicating the involvement of bcl-2 AUBPs in bcl-2 mRNA stability control. (C) 2001 Academic Press.

引用

页码：1063 / 1069

页数：7

共 22 条

[1] ARED: human AU-rich element-containing mRNA database reveals an unexpectedly diverse functional repertoire of encoded proteins [J].