Pivotal role of peroxisome proliferator-activated receptor γ (PPARγ) in regulation of erythroid progenitor cell proliferation and differentiation

被引：23

作者：

Nagasawa, E

Abe, Y

Nishimura, J

Yanase, T

Nawata, H

Muta, K

机构：

[1] Kyushu Univ, Dept Med & Bioregulatory Sci, Grad Sch Med Sci, Higashi Ku, Fukuoka 8128582, Japan

[2] Kyushu Univ, Med Inst Bioregulat, Dept Clin Immunol, Beppu, Oita, Japan

来源：

EXPERIMENTAL HEMATOLOGY | 2005年 / 33卷 / 08期

关键词：

D O I：

10.1016/j.exphem.2005.05.003

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Objective. The aim of this study was to reveal the role of peroxisome proliferator-activated receptor gamma (PPAR gamma) in erythropoiesis. Methods. The effects of PPAR gamma ligands on cellular proliferation and differentiation were investigated in erythroid colony-forming cells (ECFCs) purified from human peripheral blood. Results. RT-PCR analysis revealed that PPAR gamma mRNA is expressed in ECFCs. Synthetic PPAR gamma ligands, troglitazone or pioglitazone, suppressed cellular proliferation without inducing apoptosis and delayed maturation of ECFCs, as determined by flow cytometry. The delay in erythroid maturation by troglitazone was confirmed by the down-regulation of gamma-globin, beta-globin and GATA-1 mRNA, and the maintenance of GATA-2 mRNA. Conclusions. Our results suggest that PPAR gamma modulates the differentiation process of erythroid progenitor cells, and plays a crucial role in regulating the balance of hematopoiesis. (c) 2005 International Society for Experimental Hematology. Published by Elsevier Inc.

引用

页码：857 / 864

页数：8

共 44 条

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