Sec6 mutations and the Drosophila exocyst complex

被引：52

作者：

Murthy, M

Ranjan, R

Denef, N

Higashi, MEL

Schupbach, T

Schwarz, TL ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Childrens Hosp, Div Neurosci, Boston, MA 02115 USA

[2] Princeton Univ, Dept Mol Biol, Howard Hughes Med Inst, Princeton, NJ 08544 USA

来源：

JOURNAL OF CELL SCIENCE | 2005年 / 118卷 / 06期

关键词：

Sec6; Sec5; Sec8; oogenesis; membrane trafficking;

D O I：

10.1242/jcs.01644

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

To allow a detailed analysis of exocyst function in multicellular organisms, we have generated sec6 mutants in Drosophila. We have used these mutations to compare the phenotypes of sec6 and sec5 in the ovary and nervous system, and we find them to be similar. We also find that Sec5 is mislocalized in sec6 mutants. Additionally, we have generated an epitope-tagged Sec8 that localized with Sec5 on oocyte membranes and was mislocalized in sec5 and sec6 germ-line clones. This construct further revealed a genetic interaction of sec8 and sec5. These data, taken together, provide new information about the organization of the exocyst complex and suggest that Sec5, Sec6 and Sec8 act as a complex, each member dependent on the others for proper localization and function.

引用

收藏

页码：1139 / 1150

页数：12

相关论文

共 32 条

[21] IDENTIFICATION OF 23 COMPLEMENTATION GROUPS REQUIRED FOR POST-TRANSLATIONAL EVENTS IN THE YEAST SECRETORY PATHWAY [J].

NOVICK, P ;

FIELD, C ;

SCHEKMAN, R .

CELL, 1980, 21 (01) :205-215

[22] ARF6 controls post-endocytic recycling through its downstream exocyst complex effector [J].

Prigent, M ;

Dubois, T ;

Raposo, G ;

Derrien, V ;

Tenza, D ;

Rossé, C ;

Camonis, J ;

Chavrier, P .

JOURNAL OF CELL BIOLOGY, 2003, 163 (05) :1111-1121

[23]

Robinson NCG, 1999, MOL CELL BIOL, V19, P3580

[24] NMDA receptor trafficking through an interaction between PDZ proteins and the exocyst complex [J].

Sans, N ;

Prybylowski, K ;

Petralia, RS ;

Chang, K ;

Wang, YX ;

Racca, C ;

Vicini, S ;

Wenthold, RJ .

NATURE CELL BIOLOGY, 2003, 5 (06) :520-530

[25] The exocyst complex binds the small GTPase RaIA to mediate filopodia formation [J].

Sugihara, K ;

Asano, S ;

Tanaka, K ;

Iwamatsu, A ;

Okawa, K ;

Ohta, Y .

NATURE CELL BIOLOGY, 2002, 4 (01) :73-78

[26] The Exocyst is a multiprotein complex required for exocytosis in Saccharomyces cerevisiae [J].

TerBush, DR ;

Maurice, T ;

Roth, D ;

Novick, P .

EMBO JOURNAL, 1996, 15 (23) :6483-6494

[27] SEC6, SEC8, AND SEC15 ARE COMPONENTS OF A MULTISUBUNIT COMPLEX WHICH LOCALIZES TO SMALL BUD TIPS IN SACCHAROMYCES-CEREVISIAE [J].

TERBUSH, DR ;

NOVICK, P .

JOURNAL OF CELL BIOLOGY, 1995, 130 (02) :299-312

[28] The exocyst complex associates with microtubules to mediate vesicle targeting and neurite outgrowth [J].

Vega, IE ;

Hsu, SC .

JOURNAL OF NEUROSCIENCE, 2001, 21 (11) :3839-3848

[29] Sec2p mediates nucleotide exchange on Sec4p and is involved in polarized delivery of post-Golgi vesicles [J].

WalchSolimena, C ;

Collins, RN ;

Novick, PJ .

JOURNAL OF CELL BIOLOGY, 1997, 137 (07) :1495-1509

[30] Mechanism of recruiting Sec6/8 (exocyst) complex to the apical junctional complex during polarization of epithelial cells [J].

Yeaman, C ;

Grindstaff, KK ;

Nelson, WJ .

JOURNAL OF CELL SCIENCE, 2004, 117 (04) :559-570

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