An enzyme-linked immunosorbent assay (ELISA) for the detection of Vibrio harveyi in penaeid shrimp and water

被引：31

作者：

Robertson, PAW

Xu, HS

Austin, B ^{[1
]}

机构：

[1] Heriot Watt Univ, Dept Biol Sci, Edinburgh EH14 4AS, Midlothian, Scotland

[2] Ocean Univ Qingdao, Dept Marine Biol, Shandong 266003, Peoples R China

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 1998年 / 34卷 / 01期

关键词：

ELISA; penaeids; shrimps; Vibrio harveyi; Western blots;

D O I：

10.1016/S0167-7012(98)00066-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Plate and dipstick enzyme-linked immunosorbent assays (ELISA) were developed for the rapid detection of Vibrio harveyi from penaeid shrimp and water. The ELISA, which incorporated a polyclonal antiserum produced in a female New Zealand white rabbit, detected 10(5) cells of V. harveyi/ml. Also, the systems detected V. harveyi in water from Chinese shrimp hatcheries. The systems permitted the recognition of a wide range of V. harveyi isolates, but not those of other taxa. Western blot analysis of bacterial outer membrane proteins (OMP) indicated that a wide epitope was recognised, with many immunoreactive bands in common between isolates of V. harveyi. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：31 / 39

页数：9