Transmembrane protein topology mapping by the substituted cysteine accessibility method (SCAM™):: Application to lipid-specific membrane protein topogenesis

被引：116

作者：

Bogdanov, M ^{[1
]}

Zhang, W ^{[1
]}

Xie, J ^{[1
]}

Dowhan, W ^{[1
]}

机构：

[1] Univ Texas, Dept Biochem & Mol Biol, Sch Med, Houston, TX 77030 USA

来源：

METHODS | 2005年 / 36卷 / 02期

关键词：

membrane protein; cysteine scanning; maleimides; phospholipid; topogenesis; phoshatidylethanolamine; protein topology;

D O I：

10.1016/j.ymeth.2004.11.002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We provide an overview of lipid-dependent polytopic membrane protein topogenesis. with particular emphasis oil Escherichia coli strains genetically altered in their lipid composition and strategies for experimentally determining the transmembrane organization of proteins. A variety of reagents and experimental strategies are described including file use of lipid mutants and thiol-specific chemical reagents to Study lipid-dependent and host-specific membrane protein topogenesis by Substituted cysteine site-directed chemical labeling. Employing strains in which lipid composition can be controlled temporally during membrane protein synthesis and assembly provides a means to observe dynamic changes in protein topology its it function of membrane lipid composition. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：148 / 171

页数：24

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