Transmembrane protein topology mapping by the substituted cysteine accessibility method (SCAM™):: Application to lipid-specific membrane protein topogenesis

被引：116

作者：

Bogdanov, M ^{[1
]}

Zhang, W ^{[1
]}

Xie, J ^{[1
]}

Dowhan, W ^{[1
]}

机构：

[1] Univ Texas, Dept Biochem & Mol Biol, Sch Med, Houston, TX 77030 USA

来源：

METHODS | 2005年 / 36卷 / 02期

关键词：

membrane protein; cysteine scanning; maleimides; phospholipid; topogenesis; phoshatidylethanolamine; protein topology;

D O I：

10.1016/j.ymeth.2004.11.002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We provide an overview of lipid-dependent polytopic membrane protein topogenesis. with particular emphasis oil Escherichia coli strains genetically altered in their lipid composition and strategies for experimentally determining the transmembrane organization of proteins. A variety of reagents and experimental strategies are described including file use of lipid mutants and thiol-specific chemical reagents to Study lipid-dependent and host-specific membrane protein topogenesis by Substituted cysteine site-directed chemical labeling. Employing strains in which lipid composition can be controlled temporally during membrane protein synthesis and assembly provides a means to observe dynamic changes in protein topology its it function of membrane lipid composition. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：148 / 171

页数：24

共 144 条

[71] Chaperone action in the posttranslational topological reorientation of the hepatitis B virus large envelope protein: Implications for translocational regulation [J].

Lambert, C ;

Prange, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (09) :5199-5204

[72] Dual topology of the hepatitis B virus large envelope protein - Determinants influencing post-translational pre-S translocatton [J].

Lambert, C ;

Prange, R .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (25) :22265-22272

[73] Transmembrane topography of the 100-kDa a subunit (Vph1p) of the yeast vacuolar proton translocating ATPase [J].

Leng, XH ;

Nishi, T ;

Forgac, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (21) :14655-14661

[74]

Levy D, 1996, Essays Biochem, V31, P49

[75] P-glycoprotein misfolds in Escherichia coli:: evidence against alternating-topology models of the transport cycle [J].

Linton, KJ ;

Higgins, CF .

MOLECULAR MEMBRANE BIOLOGY, 2002, 19 (01) :51-58

[76]

LIU XY, 2002, BIOCHIM BIOPHYS ACTA, V31, P333

[77] Characterization of the first cytoplasmic loop of subunit a of the Escherichia coli ATP synthase by surface labeling, cross-linking, and mutagenesis [J].

Long, JC ;

DeLeon-Rangel, J ;

Vik, SB .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (30) :27288-27293

[78] Membrane topology of subunit a of the F1F0 ATP synthase as determined by labeling of unique cysteine residues [J].

Long, JC ;

Wang, S ;

Vik, SB .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (26) :16235-16240

[79] MEMBRANE TOPOLOGY OF A CYSTEINE-LESS MUTANT OF HUMAN P-GLYCOPROTEIN [J].

LOO, TW ;

CLARKE, DM .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (02) :843-848

[80] Reorientation of aquaporin-1 topology during maturation in the endoplasmic reticulum [J].

Lu, Y ;

Turnbull, IR ;

Bragin, A ;

Carveth, K ;

Verkman, AS ;

Skach, WR .

MOLECULAR BIOLOGY OF THE CELL, 2000, 11 (09) :2973-2985

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